阿魏酸对CT-26的细胞凋亡和细胞自噬相关通路的影响  

The effects of ferulic acid on the apoptosis and autophagy-related pathways in CT-26 cells

作　　者：陈杉彬 赵东 栾春光 乔宗伟 郑佳 张强 冯政 王德良 CHEN Shanbin;ZHAO Dong;LUAN Chunguang;QIAO Zongwei;ZHENG Jia;ZHANG Qiang;FENG Zheng;WANG Deliang(China National Research Institute of Food and Fermentation Industries,Beijing 100015,China;Yibin Wuliangye Group Limited.,Yibin 644000,China;International Joint Research Center of Quality and Safety of Alcoholic Beverages,Beijing 100015,China)

机构地区：[1]中国食品发酵工业研究院有限公司,北京100015 [2]宜宾五粮液股份有限公司,四川宜宾644000 [3]酒类品质与安全国际联合研究中心,北京100015

出　　处：《食品与发酵工业》2024年第6期367-371,共5页Food and Fermentation Industries

基　　金：中国轻工集团科技创新基金项目(ZQ2022YY03)。

摘　　要：为了深入明晰酚酸类物质阿魏酸的具体功能机理,以其对CT-26细胞自噬、凋亡、细胞增殖和迁移为评价手段,考察阿魏酸对细胞生理活性的影响,阐明酚酸类物质阿魏酸具体作用机理,为中草药中有效成分阿魏酸的具体功效提供实验数据和理论依据。研究中采用不同浓度梯度的阿魏酸处理CT-26细胞,考察阿魏酸对CT-26细胞生理活性的影响。在一定浓度范围内,阿魏酸能够抑制CT-26细胞的增殖、提高细胞自噬水平,同时在一定程度上抑制CT-26细胞的迁移能力。进一步的研究表明,阿魏酸通过促进凋亡通路上游的caspase3蛋白剪切为Cleaved caspase3,进而调控凋亡关键蛋白Bcl-2和BCL2-Associated X蛋白(Bax)的比值,最终促进了结肠癌细胞凋亡,以此保护细胞的正常生理活动。阿魏酸可通过促进细胞自噬并调控凋亡通路Bcl-2来抑制细胞活性,进而促进细胞凋亡。研究结果为明确中草药中有效成分阿魏酸的生物活性物质作用提供了实验数据。This study aimed to investigate the specific mechanism of action of the phenolic acid compound ferulic acid.The impact of ferulic acid on cell physiological activity was investigated by using its effects on CT-26 cell autophagy,apoptosis,cell proliferation,and migration as evaluation methods.This study provides experimental data and theoretical basis for the specific efficacy of the active ingredient ferulic acid in traditional Chinese medicine.Colon cancer cell line CT-26 was treated with different concentrations of ferulic acid(FA)to study whether ferulic acid could inhibit CT-26 cell.Within a certain concentration range,ferulic acid could inhibit the proliferation of CT-26 cells,slightly increase the level of autophagy,and inhibit the migration of CT-26 cells to a certain extent.In addition,ferulic acid could promote the cleavage of caspase-3 protein into cleaved caspase-3 protein,and then regulated the proportion of apoptosis Bcl-2 and Bax,so as to promote the apoptosis of colon cancer cells and protect the normal physiological activities of the body.Ferulic acid may inhibit tumor activity and promote cancer cell apoptosis by promoting autophagy and regulating apoptosis pathway bcl-2.The results provided experimental evidence for the bioactive substance of ferulic acid in Chinese herbal medicine.

关 键 词：阿魏酸 CT-26细胞 细胞自噬 凋亡 

分 类 号：TS201.4[轻工技术与工程—食品科学]