罗平小黄姜总黄酮提取工艺的优化及抗氧化活性研究  

Optimization of Extraction Process of Total Flavonoids from Luoping Zingiber offcinale Roscoe and Study on Its Antioxidant Activity

作　　者：刘静 刘继华 叶朋飞 李秋达 张春艳 黄慧福[2] LIU Jing;LIU Jihua;YE Pengfei;LI Qiuda;ZHANG Chunyan;HUANG Huifu(Qujing Vocational and Technical College,Qujing 655011;Qujing Normal University,Qujing 655011)

机构地区：[1]曲靖职业技术学院,曲靖655011 [2]曲靖师范学院,曲靖655011

出　　处：《食品工业》2024年第2期64-68,共5页The Food Industry

基　　金：云南省教育厅科学研究基金项目(2023J2072)。

摘　　要：小黄姜是一种药食两用的经济作物,含有多种生物活性物质。试验以罗平小黄姜为原料,对小黄姜总黄酮的提取工艺及抗氧化活性进行研究。以液料比、提取温度、提取时间、乙醇体积分数为单因素,总黄酮提取率为评价指标进行单因素试验及正交试验,确定最佳提取工艺条件。以VC作为对照,探究小黄姜总黄酮提取液对DPPH·、ABTS^(+)·自由基的清除作用。结果表明,罗平小黄姜总黄酮提取最佳工艺条件为液料比25︰1(mL/g)、提取温度80℃、提取时间2.5 h、乙醇体积分数65%,在此工艺条件下,小黄姜总黄酮的提取率为4.959%。小黄姜总黄酮提取液对DPPH·、ABTS^(+)·自由基的最大清除率分别为79.27%和91.26%。该方法操作简便,可用于小黄姜中总黄酮的提取,且其提取液具有一定的自由基清除作用。Zingiber oficinale Roscoe is the cash crop which can be used as medicine and food,and it has been connected with many bioactive substances.The Luoping Zingiber oficinale Roscoe was used as materials,and the extraction process and antioxidant activity of total flavonoids from ginger were studied.The optimal extraction conditions were determined by single factor test and orthogonal test with liquid-solid ratio,extraction temperature,extraction time and ethanol concentration as single factor and total flavonoids extraction rate as evaluation index.The scavenging effect of extract was analyzed by DPPH.and ABTS^(+),in which VC was used as the control.The results showed that the optimum extraction condition was as follows:liquid-solid ratio 25:1(g/mL),extraction temperature 80℃,extraction time 2.5 h and ethanol concentration 65%,and the extration rate of total flavonoids from Luoping Zingiber oficinale Roscoe was 4.959%.The maximum scavenging rates of DPPH:and ABTS^(+)radicals by the extract were 79.27%and 91.26%,respectively.The method is simple and can be used for the extraction of total flavonoids from Zingiber officinale Roscoe,and its extract has certain free radical scavenging effect.

关 键 词：小黄姜 总黄酮 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]