欧李种质资源自交不亲和S-RNase基因的克隆及序列分析  

Cloning and sequence analysis of self-incompatibility S--RNase genes from Prunus humilis germplasm resources

作　　者：郭夕雯 穆霄鹏[1] 王鹏飞[1] 张建成[1] 张帅 杜俊杰[1] GUO Xiwen;MU Xiaopeng;WANG Pengfei;ZHANG Jiancheng;ZHANG Shuai;DU Junjie(Shanxi Agricultural University College of Horticulture,Jinzhong 030801)

机构地区：[1]山西农业大学园艺学院,晋中030801

出　　处：《中国果树》2024年第2期38-43,共6页China Fruits

基　　金：山西农业大学学术恢复科研专项(2020xshf22、2020xshf23)。

摘　　要：为鉴定不同欧李种质资源的S基因型,分析欧李S基因序列特点,以70份欧李种质为材料,通过PCR特异性扩增S基因,并克隆测序得到其基因序列。结果表明:利用BFP93/BFP94-1引物,在52份种质中各鉴定到2个S基因,在16份种质中各鉴定到单个S基因,在3-32-扁黄和10-32两份种质中未扩增出条带;共克隆得到120条基因,鉴定得到36种S基因型,并将其中的新基因登录到NCBI中,登录号依次为OQ124075~OQ124109。The objective of this study was to identify the S genotypes of different Prunushumilis germplasm resources and analyze the sequence characteristics of the Prunushumilis S gene.A total of 70 Prunushumilis germplasm resources were used as test materials.The S gene was amplified through PCR,followed by cloning and sequencing to obtain its gene sequence.Using the BFP93/BFP94-1 primers,two S alleles were identified in each of the 52 germplasm resources.Single S allele was identified in 16 germplasm resources.No bands were amplified in the‘3-32-bianhuang’and‘10-32’germplasm resources.A total of 120 genes were cloned,and 36 S genotypes were identified.The new genes were registered in NCBI with registration numbers in the following sequence OQ124075~OQ124109.

关 键 词：欧李 S基因 自交不亲和 特异性引物 

分 类 号：S662.5[农业科学—果树学]