一种可快速检测大肠杆菌O157:H7的纸基传感器的制备与应用  

Preparation and Application of A Paper Sensor for Rapid Detection of Escherichia coli O157:H7

作　　者：王晓颖 施锦辉 王金娟 戴晗祎 郭骁驹 王晓惠 WANG Xiaoying;SHI Jinhui;WANG Jinjuan;DAI Hanyi;GUO Xiaoju;WANG Xiaohui(Nantong Customs Comprehensive Technology Center,Nantong 226004,China;School of Food Science and Technology,Wuxi 214122,China;Nantong Rugao Ecological Environment Monitoring Station,Rugao 226500,China)

机构地区：[1]南通海关综合技术中心,江苏南通226004 [2]江南大学食品学院,江苏无锡214122 [3]南通市如皋生态环境监测站,江苏如皋226500

出　　处：《现代食品》2024年第3期173-177,共5页Modern Food

基　　金：南京海关科研项目(2022KJ27)。

摘　　要：大肠杆菌O157:H7是一种致病性较强的食源性细菌,通常在生制食品中被检出,一旦被人体摄入会引发肠道疾病,严重危害公众健康。本研究制备了功能化的长余辉探针,并结合适配体的特异性识别功能构建了能快速检测大肠杆菌O157:H7的纸基传感器,检出限达7.5×10^(3) CFU·mL^(-1)。该传感器可同时检测3个样品,具有特异性好、操作简便和环境友好的特点,可应用于大量样品中大肠杆菌O157:H7的快速筛查。Escherichia coli O157:H7 is a highly pathogenic foodborne bacteria,usually detected in raw foods,once ingested by humans can cause intestinal disease,a serious threat to public health.In this study,a functionalized long afterglow probe was prepared,and a paper sensor capable of rapid detection of Escherichia coli O157:H7 was constructed with a detection limit of 7.5×10^(3) CFU·mL^(-1) combined with the specific recognition function of aptamer.The sensor can detect three samples simultaneously,with good specificity,easy operation and environmental friendliness.It can be applied to the rapid screening of Escherichia coli O157:H7 in a large number of samples.

关 键 词：大肠杆菌O157:H7 长余辉探针 纸基传感器 快速筛查 

分 类 号：TS207.4[轻工技术与工程—食品科学]