江香薷炮制前后多糖含量及消炎、止血和抗氧化活性的影响  

Effects of polysaccharide content and anti⁃inflammatory,hemostatic,and antioxidant activities before and after preparation of Mosla chinensis⁃Jiangxiangru

作　　者：马秋婷 徐磊 朱钰晨 徐梦婷 张文凯 刘志勇[1] MA Qiuting;XU Lei;ZHU Yuchen;XU Mengting;ZHANG Wenkai;LIU Zhiyong(Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学,南昌330004

出　　处：《中国比较医学杂志》2024年第2期79-88,共10页Chinese Journal of Comparative Medicine

基　　金：江西中医药大学校级科技创新团队发展计划(CXTD⁃22004);江西中医药大学省级创新创业训练计划(S202310412055)。

摘　　要：目的探究生姜汁炮制前后江香薷多糖含量变化,以及对炮制前后多糖消炎止血抗氧化活性评价。方法利用苯酚-硫酸法测定炮制前后江香薷多糖(JXRPs)和姜制江香薷多糖(JZJXRPs)的含量;体内通过构建大鼠足肿胀模型和体外内毒素(LPS)诱导小鼠巨噬细胞RAW264.7炎症模型,采用细胞增殖法(MTT)选取最佳给药浓度,酶联免疫吸附试验(ELISA)测定细胞上清液中白介素-6(IL⁃6)、白介素-12(IL⁃12)、一氧化氮(NO)、白介素-4(IL⁃4)和白介素-10(IL⁃10)的表达来评价炮制前后多糖的抗炎作用;通过剪尾法观察小鼠的出血时间来评价其止血效果;最后,利用清除1,1-二苯基-2-苦基肼(DPPH)和2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS)的能力来评价其体外抗氧化活性能力。结果生姜汁炮制前后江香薷多糖的含量分别为13%、22%;大鼠足肿胀结果显示,在注射角叉菜胶4 h后,与模型组相比,江香薷多糖在给药剂量为200 mg/kg出现显著性差异(P<0.05),而姜制江香薷多糖在给药剂量为100 mg/kg时就可明显减轻大鼠足肿胀(P<0.05);体外抗炎结果显示,炮制前后的多糖均能显著抑制细胞分泌IL⁃6、IL⁃12和NO(P<0.01),促进细胞分泌IL⁃4和IL⁃10(P<0.01),且炮制后作用更强。小鼠断尾止血实验结果表明,与正常组相比,江香薷多糖组可以加快止血效果但无显著性差异,而姜制江香薷多糖在中剂量时有显著性差异(P<0.05),而高剂量有加快趋势但并无显著性差异;体外抗氧化活性结果显示炮制前后的江香薷多糖对DPPH和ABTS皆有不同程度的清除能力,其中江香薷多糖的IC_(50)值分别为0.2215 mg/mL和0.2110 mg/mL,姜制江香薷多糖的IC_(50)值分别为0.1651 mg/mL和0.1884 mg/mL。结论江香薷经生姜汁炮制后可促进多糖的溶出,增加多糖含量;炮制后江香薷多糖消炎止血及抗氧化能力均比江香薷多糖强,可为江香薷多糖后续研究及临床应用奠定基础�Objective Explore changes in polysaccharides in Jiangxiangru before and after ginger juice preparation,and evaluate polysaccharide anti⁃inflammatory and antioxidant activities before and after processing.Methods The contents of Jiangxiangru polysaccharide(JXRPs)and Ginger juice processed of Jiangxiangru polysaccharide(JZJXRPs)before and after processing were determined by phenol⁃sulfuric acid method.We used the swelling model in rats and endotoxin(LPS)to establish the RAW264.7 mouse macrophage inflammation model.The optimal administration concentration was determined using a cell proliferation(MTT)assay.Enzyme⁃linked immunosorbent assay(ELISA)were used to measure Interleukin⁃6(IL⁃6),Interleukin⁃12(IL⁃12),Nitric oxide(NO),Interleukin⁃4(IL⁃4),and Interleukin⁃10(IL⁃10).Bleeding time of mice by tail cutting was observed to evaluate the hemostatic effect.The ability to remove 1,1⁃diphenyl⁃2⁃picryl⁃hydrazyl radical(DPPH)and 2,2’⁃Azinobis⁃(3⁃ethylbenzthiazoline⁃6⁃sulphonate)(ABTS)was used to evaluate in vitro antioxidant activity.Results The contents of JXRPs and JZJXRPs were 13%and 22%,respectively.In swollen rats at 4 h after injection,compared with the model group,200 mg/kg JXRPs and 100 mg/kg JZJXRPs significantly reduced rat swelling(P<0.05).In vitro anti⁃inflammation assessment showed that the polysaccharides before and after processing significantly inhibited secretion of IL⁃6,IL⁃12,and NO(P<0.01)and promoted secretion of IL⁃4 and IL⁃10(P<0.01),and also that processing post⁃effects were stronger.The hemostatic experiment show that,compared with the control group,JXRPs increased hemostasis,but without a significant difference,and no significant difference was found using JZJXRPs,although high doses showed a trend to increase hemostasis.In vitro antioxidant activity showed that JXRPs and JZJXRPs had different scavenging abilities for DPPH and ABTS with IC_(50) values of JXRPs of 0.2215 and 0.2110 mg/ml,respectively,and IC_(50) values of JZJXRPs of 0.1651 and 0.1

关 键 词：江香薷 炮制 多糖 消炎止血 抗氧化活性 

分 类 号：R-33[医药卫生]