酱香型白酒中2,3-丁二酮气相色谱检测方法研究  

Detection of 2,3-Butanedione in Jiangxiang Baijiu by Gas Chromatography

作　　者：彭禹熙 樊双喜 张娅 李细芬 尹学忠 王国江 杨礼红 侯玉祥 卢君 李长文 PENG Yuxi;FAN Shuangxi;ZHANG Ya;LI Xifen;YIN Xuezhong;WANG Guojiang;YANG Lihong;HOU Yuxiang;LU Jun;LI Changwen(Guizhou Guotai Distillery Co.Ltd.,Renhuai,Guizhou 564501;Guotai Research Academy,Tianjin 300410,China)

机构地区：[1]贵州国台酒业集团股份有限公司,贵州仁怀564501 [2]贵州国台酒业集团研究院,天津300410

出　　处：《酿酒科技》2024年第3期108-112,117,共6页Liquor-Making Science & Technology

基　　金：贵州省科技成果应用及产业化计划项目(黔科合成果[2020]2Y045);遵义市科技计划项目(遵市科合R&D[2020]31号);遵义市科技计划项目(遵市科合支撑GY(2021)40号)。

摘　　要：基于直接进样结合气相色谱(GC)技术,通过优化确认检测方法条件(即柱流速1 mL/min;分流比1∶20;柱温箱升温程序:初始柱温箱40℃,保持2 min,以3℃/min升温至90℃,再以15℃/min升温至230℃,保持20 min),研究建立了GC外标法定量分析检测酱香型白酒中2,3-丁二酮的方法。结果表明,该方法在质量浓度7.05~117.5 mg/L范围内线性关系良好(R2=0.998);检出限为0.67 mg/L(S/N=3),定量限为2.24 mg/L(S/N=10);加标回收率范围在82.77%~112.61%之间;日内与日间RSD分别为0.41%和1.44%。该方法操作简便、准确性高、重复性好,可以广泛推广应用于酱香白酒中2,3-丁二酮含量测定。Based on direct injection method and gas chromatography(GC),the quantitative analysis method of 2,3-butanedione in Ji-angxiang Baijiu was established,and the detection conditions were optimized as follows:the column flow rate was 1 mL/min,the split ratio was 1∶20,the initial column temperature was 40℃(maintain for 2 min),the temperature rise rate was 3℃/min to 90℃,and 15℃/min to 230℃(maintain for 20 min).The results showed that the method had good linearity in the range of 7.05 mg/L to 117.5 mg/L(R2=0.998),the detection limit was 0.67 mg/L(S/N=3),the quantification limit was 2.24 mg/L(S/N=10),the recovery rate was between 82.77%and 112.61%,and the intra-day and inter-day relative standard deviation was 0.41%and 1.44%,respec-tively.This method is simple to operate,highly accurate,and exhibits good repeatability,making it suitable for the determination of 2,3-butanedione in Jiangxiang Baijiu.

关 键 词：酱香型白酒 2 3-丁二酮 气相色谱 方法优化 方法学 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]