植物乳植杆菌J26组合物对高脂饮食诱导肥胖小鼠的减肥作用研究  

Study on the weight loss effect of Lactiplantibacillus plantarum J26 combination on high-fat diet-induced obese mice

作　　者：郑亚平 郝占西 姜毓君[1] 满朝新[1] 张宇[1] ZHENG Yaping;HAO Zhanxi;JIANG Yujun;MAN Chaoxin;ZHANG Yu(Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agricultural University,Harbin 150030,China;Junyao Runying Biotechnology(Shanghai)Co.Ltd.,Shanghai 201799,China;National Dairy Engineering Technology Research Centre,Shanghai 201799,China)

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]均瑶润盈生物科技(上海)有限公司,上海201799 [3]国家乳业工程技术研究中心润盈(上海)实验室,上海201799

出　　处：《中国乳品工业》2024年第3期5-13,64,共10页China Dairy Industry

基　　金：黑龙江省自然科学基金联合引导项目(LH2023C033)。

摘　　要：胞外多糖是一类来源广泛的生物活性分子,具有调节肠道菌群的潜力。文章利用单因素及响应面优化实验确定副干酪乳酪杆菌(副干酪乳杆菌)JY56胞外多糖的最优发酵条件:发酵温度为30℃,发酵时间为26 h,发酵pH为6.0。进一步制备了植物乳植杆菌J26与副干酪乳酪杆菌JY56胞外多糖组合物(J26&JY56 Exopolysaccharide Composition, JEC)。为了研究组合物JEC对肥胖的影响,采用高脂饮食诱导建立肥胖小鼠模型。经饮食干预10周后,结果表明,组合物JEC减缓体重增加的效果显著(P<0.05),缓解了由于长期摄入高脂饮食造成的肝脏脂肪堆积,基本恢复到正常水平。其中,JEC组小鼠血清中的TNF-α(301.77±9.87) ng/L、IL-6(225.39±10.09) ng/L、IL-1β(21.92±1.58) ng/L和IFN-γ(180.90±8.52)ng/L含量明显低于高脂饮食诱导的肥胖模型小鼠组(HFD)(P<0.05)。此外,JEC组的肝细胞中未观察到明显的脂滴空泡和坏死细胞,以及炎症细胞浸润现象。植物乳植杆菌J26与副干酪乳酪杆菌JY56胞外多糖组合物JEC具有对高脂饮食诱导的肥胖小鼠的减肥作用。Polysaccharides are a class of bioactive molecules from a wide range of sources that have the potential to regulate intestinal microbiota.In this study,the optimal fermentation conditions for exopolysaccharide of Lacticaseibacillus paracasei JY56 were determined using single-factor and response surface optimization experiment:the fermentation temperature was 30°C,the fermentation time was 26 h,and the fermentation pH was 6.0.A combination of Lactiplantibacillus plantarum J26 and Lacticaseibacillus paracasei JY56 exopolysaccharide(J26&JY56 Exopolysaccharide Composition,JEC)was prepared.To study the effect of JEC on obesity,a high-fat diet was used to establish an obese mouse model.After 10 weeks of dietary intervention,the results showed that JEC was effective in slowing down weight gain(P<0.05),alleviating liver fat accumulation due to chronic intake of high-fat diet,and largely returning to normal levels.Specifically,the serum levels of TNF-α(301.77±9.87)ng/L,IL-6(225.39±10.09)ng/L,IL-1β(21.92±1.58)ng/L and IFN-γ(180.90±8.52)ng/L in the JEC group mice were significantly lower than those in the high fat diet induced obesity model mice group(HFD)(P<0.05).In addition,no significant lipid droplet vacuoles and necrotic cells were observed in the hepatocytes of the JEC group,and no inflammatory cell infiltration was observed.A combination of Lactiplantibacillus plantarum J26 and Lacticaseibacillus paracasei JY56 exopolysaccharides had positive modulatory effects on delaying high-fat diet-induced obesity in mice.

关 键 词：植物乳植杆菌J26 副干酪乳酪杆菌JY56胞外多糖 组合物 合生素 肥胖 

分 类 号：Q93-31[生物学—微生物学]