Humanβ-defensin-1 affects the mammalian target of rapamycin pathway and autophagy in colon cancer cells through long noncoding RNA TCONS_00014506  

作　　者：Yu-Xin Zhao Yan Cui Xin-Hong Li Wen-Hong Yang Shi-Xiang An Jia-Xian Cui Min-Yu Zhang Jing-Kun Lu Xuan Zhang Xiu-Mei Wang Li-Li Bao Peng-Wei Zhao 

机构地区：[1]Department of Anesthesiology,Inner Mongolia Chest Hospital,The Fourth Hospital,Hohhot 010035,Inner Mongolia Autonomous Region,China [2]College of Humanities and Education,Inner Mongolia Medical University,Hohhot 010059,Inner Mongolia Autonomous Region,China [3]Department of Radiotherapy,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010059,Inner Mongolia Autonomous Region,China [4]School of Basic Medical Science,Inner Mongolia Medical University,Hohhot 010059,Inner Mongolia Autonomous Region,China [5]Department of Medical Oncology,The Affiliated Cancer Hospital of Inner Mongolia Medical University,Hohhot 010050,Inner Mongolia Autonomous Region,China

出　　处：《World Journal of Gastrointestinal Oncology》2024年第4期1465-1478,共14页世界胃肠肿瘤学杂志（英文版）（电子版）

基　　金：Supported by National Natural Science Foundation of China,No.82360329;Inner Mongolia Medical University General Project,No.YKD2023MS047;Inner Mongolia Health Commission Science and Technology Plan Project,No.202201275.

摘　　要：BACKGROUND Colorectal cancer has a low 5-year survival rate and high mortality.Humanβ-defensin-1(hBD-1)may play an integral function in the innate immune system,contributing to the recognition and destruction of cancer cells.Long non-coding RNAs(lncRNAs)are involved in the process of cell differentiation and growth.AIM To investigate the effect of hBD-1 on the mammalian target of rapamycin(mTOR)pathway and autophagy in human colon cancer SW620 cells.METHODS CCK8 assay was utilized for the detection of cell proliferation and determination of the optimal drug concentration.Colony formation assay was employed to assess the effect of hBD-1 on SW620 cell proliferation.Bioinformatics was used to screen potentially biologically significant lncRNAs related to the mTOR pathway.Additionally,p-mTOR(Ser2448),Beclin1,and LC3II/I expression levels in SW620 cells were assessed through Western blot analysis.RESULTS hBD-1 inhibited the proliferative ability of SW620 cells,as evidenced by the reduction in the colony formation capacity of SW620 cells upon exposure to hBD-1.hBD-1 decreased the expression of p-mTOR(Ser2448)protein and increased the expression of Beclin1 and LC3II/I protein.Furthermore,bioinformatics analysis identified seven lncRNAs(2 upregulated and 5 downregulated)related to the mTOR pathway.The lncRNA TCONS_00014506 was ultimately selected.Following the inhibition of the lncRNA TCONS_00014506,exposure to hBD-1 inhibited p-mTOR(Ser2448)and promoted Beclin1 and LC3II/I protein expression.CONCLUSION hBD-1 inhibits the mTOR pathway and promotes autophagy by upregulating the expression of the lncRNA TCONS_00014506 in SW620 cells.

关 键 词：Colon cancer Humanβ-defensin-1 LncRNA Mammalian target of rapamycin AUTOPHAGY 

分 类 号：R735.7[医药卫生—肿瘤]