一起副溶血性弧菌引起的群体性食物中毒事件溯源分析  

Source tracing analysis of a cluster food poisoning incident caused by Vibrio parahaemolyticus

作　　者：庄春红 吴小凤[3] 郑友限[3] ZHUANG Chunhong;WU Xiaofeng;ZHENG Youxian(Quanzhou Medical College,Quanzhou,Fujian 362000,China;Fujian Provincial University Applied Technology Engineering Center for Food Safety Testing,Quanzhou,Fujian 362000,China;Quanzhou Center for Disease Control and Prevention,Quanzhou,Fujian 362000,China)

机构地区：[1]泉州医学高等专科学校,福建泉州362000 [2]食品安全检测福建省高校应用技术工程中心,福建泉州362000 [3]泉州市疾病预防控制中心,福建泉州362000

出　　处：《应用预防医学》2024年第1期5-8,共4页Applied Preventive Medicine

基　　金：福建省中青年课题(科技类)(JAT201241);泉州医学高等专科学校科研课题(XJ1519A)。

摘　　要：目的 对一起副溶血性弧菌引起的群体性食物中毒事件进行溯源分析,为避免类似食物中毒事件发生提供依据。方法 对采自该食物中毒事件现场的接触者肛拭子、食物样本、环境涂抹样本进行病原学检测,并对分离出的副溶血性弧菌进行毒力基因鉴定、血清学分型和脉冲场凝胶电泳(PFGE)分子分型。结果 采集的38份样本中,有21份(19份肛拭子、2份环境涂抹样本)检出副溶血性弧菌,检出率为55.3%。分离出的21株副溶血性弧菌血清型均为O3:K6,其tlh/tdh毒力基因为阳性,PFGE分子分型显示高度同源(同源性在98.7%~100.0%之间)。结论 综合流行病学调查结果和实验室检测结果判定,引起该群体性食物中毒事件的病原菌为携带tlh/tdh基因的O3:K6型副溶血性弧菌。建议监管部门加强卫生监管力度,避免类似事件的发生。Objective To analyze the source of a cluster food poisoning incident caused by Vibrio parahaemolyticus(VP),and provide evidence for avoiding similar food poisoning events.Methods Conduct pathogenic testing on anal swabs,food samples,and environmental smear samples of contacts collected from the scene of the food poisoning incident.Virulence gene identification,serological typing,and pulsed field gel electrophoresis(PFGE)molecular typing were performed on the isolated Vibrio parahaemolyticus.Results Among the 38 samples that were collected,Vibrio parahaemolyticus was detected in 21 of the samples(19 anal swabs and 2 environmental smear samples),with a detection rate of 55.3%.The 21 isolated Vibrio parahaemolyticus serotypes were all O3:K6,and their tlh/tdh virulence genes were positive.PFGE molecular typing showed high homology(homology between 98.7% and 100.0%).Conclusions Based on the combined epidemiological investigation results and laboratory test results,it was determined that the pathogen causing the group food poisoning incident was Vibrio parahaemolyticus type O3:K6,which carrying tlh/tdh gene.It is recommended that regulatory authorities strengthen health supervision to avoid similar incidents from happening.

关 键 词：食源性疾病 副溶血性弧菌 血清型 荧光定量PCR 脉冲场凝胶电泳 

分 类 号：R516.2[医药卫生—内科学]