A comparative study of human and porcine-derived decellularised nerve matrices  

作　　者：Rui Li Shuai Qiu Weihong Yang Zilong Rao Jiaxin Chen Yuexiong Yang Qingtang Zhu Xiaolin Liu Ying Bai Daping Quan 

机构地区：[1]Guangdong Engineering Technology Research Centre for Functional Biomaterials,School of Materials Science and Engineering,Sun Yat-sen University,Guangzhou,Guangdong Province,China [2]Key Laboratory for Polymeric Composite&Functional Materials of Ministry of Education,School of Chemistry,Sun Yat-sen University,Guangzhou,Guangdong Province,China [3]Guangdong Engineering Technology Research Centre for Peripheral Nerve Tissue,Department of Orthopaedic and Microsurgery,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong Province,China [4]Guangzhou Zhongda Medical Equipment Co.,Ltd.,Guangzhou,Guangdong Province,China

出　　处：《Biomaterials Translational》2023年第3期180-195,共16页生物材料转化电子杂志（英文）

基　　金：the National Natural Science Foundation of China(Nos.51903255 and 52073314);The Key Areas Research and Development Program of Guangdong Province(No.2020B1111150003);Science and Technology Program of Guangzhou City(No.201904010364).

摘　　要：Decellularised extracellular matrix(dECM)biomaterials originating from allogeneic and xenogeneic tissues have been broadly studied in the field of regenerative medicine and have already been used in clinical treatments.Allogeneic dECMs are considered more compatible,but they have the drawback of extremely limited human tissue sources.Their availability is also restricted by the health and age of the donors.To investigate the viability of xenogeneic tissues as a substitute for human tissues,we fabricated both porcine decellularised nerve matrix(pDNM)and human decellularised nerve matrix for a comprehensive comparison.Photomicrographs showed that both dECM scaffolds retained the ECM microstructures of native human nerve tissues.Proteomic analysis demonstrated that the protein compositions of both dECMs were also very similar to each other.Their functional ECM contents effectively promoted the proliferation,migration,and maturation of primary human Schwann cells in vitro.However,pDNM contained a few antigens that induced severe host immune responses in humanised mice.Interestingly,after removing theα-galactosidase antigen,the immune responses were highly alleviated and the pre-treated pDNM maintained a human decellularised nerve matrix-like pro-regenerative phenotype.Therefore,we believe that anα-galactosidase-free pDNM may serve as a viable substitute for human decellularised nerve matrix in future clinical applications.

关 键 词：ALLOGENEIC decellularised nerve matrix immune response xenogeneic Α-GAL 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]