SPE-UPLC联用快速检测保健品中人参皂苷Rh2成分  

Rapid Determination of Ginsenoside Rh2 in Health Products by SPE-UPLC

作　　者：韩璐 张杰[1] 陈小兵 李刚[1] 李莉[1] 徐涛[1] HAN Lu;ZHANG Jie;CHEN Xiaobing;LI Gang;LI Li;XU Tao(School of Pharmacy,Qiqihar Medical College,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,黑龙江齐齐哈尔161006

出　　处：《食品安全导刊》2024年第9期69-72,76,共5页China Food Safety Magazine

基　　金：黑龙江省省属高校基本科研业务费科研项目(2021-KYYWF-0350)。

摘　　要：目的:基于固相萃取与超高液相色谱联用技术,建立一种快速准确检测保健品中人参皂苷Rh2成分的方法,为市售含有人参皂苷Rh2成分的保健品的质量评价提供参考。方法:样品预处理后经甲醇超声提取,中性氧化铝SPE小柱净化,筛选合适的洗脱剂进行HLB-SPE小柱分离纯化,采用WatersT3(100 mm×2.1 mm,1.8μm)色谱柱,乙腈-水为流动相梯度洗脱,PDA全波长扫描,流速0.3 mL·min^(-1)。结果:SPE-UPLC法测得人参皂苷Rh2浓度在9.7~99.8μg·mL^(-1)与其峰面积线性关系良好(r=0.999),平均回收率为99.20%。结论:该方法准确、简便、快速,并能有效去除辅料干扰,稳定性高,可用于含有人参皂苷Rh2有效成分的保健食品的质量控制。Objective:To establish a rapid and accurate method for the determination of rare ginsenoside Rh2 in health food products based on solid-phase extraction coupled with ultra-high liquid chromatography(UPLC),and to provide a reference for the quality evaluation of commercially available health food products containing ginsenoside Rh2.Method:The samples were pretreated and extracted by methanol ultrasonication,purified by a neutral alumina SPE column,and screened for suitable eluents for the separation and purification on a HLB-SPE column,using a WatersT3(100 mm×2.1 mm,1.8μm)chromatographic column with acetonitrile-water as the mobile phase in a gradient elution,and scanned by a PDA at full wavelength at aflow rate of 0.3 mL·min^(-1).Result:The concentration of ginsenoside Rh2 measured by SPE-UPLC showed a good linear relationship with its peak area in the range of-19.7~99.8μg·mL(r=0.999),and the average recovery was 99.20%.Conclusion:The method is accurate,simple,rapid and effective in removing the interference of excipients,with high stability,and can be used for the quality control of health food containing ginsenoside Rh2 active ingredient.

关 键 词：固相萃取 超高效液相色谱(UPLC) 保健食品 人参皂苷RH2 含量测定 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]