高效液相色谱法测定糕点和果汁中纳他霉素的方法学验证  

Methodological Validation of High Performance Liquid Chromatography for the Determination of Natamycin in Pastries and Fruit Juice

作　　者：卢薇 周美丽 LU Wei;ZHOU Meili(Baoji Food and Drug Inspection and Testing Center,Baoji 721000,China)

机构地区：[1]宝鸡市食品药品检验检测中心,陕西宝鸡721000

出　　处：《食品安全导刊》2024年第7期96-99,共4页China Food Safety Magazine

摘　　要：为了验证宝鸡市食品药品检验检测中心是否有能力开展纳他霉素新方法《食品安全国家标准食品中纳他霉素的测定》(GB 5009.286—2022)的检测项目。本文使用高效液相色谱仪,对糕点和果汁中纳他霉素进行分析,外标法定量,验证了本方法的线性关系、方法检出限、精密度和回收率。结果表明,纳他霉素在0.5~10.0μg·mL^(-1)呈现良好的线性关系,方法检出限、精密度和回收率均能达到标准方法要求。因此,本实验室具备检测纳他霉素的能力。In order to verify whether the Baoji Food and Drug Inspection and Testing Center has the ability to carry out the testing project of the new method GB 5009.286—2022 for natamycin.This article uses a high-performance liquid chromatography to analyze natamycin in pastries and fruit juice.The external standard method is used for quantification to verify the linear relationship,detection limit,precision,and recovery rate of this-1 method.The results showed that natamycin exhibited a good linear relationship within the range from 0.5μg·mL to 10μg·mL^(-1),and the detection limit,precision,and recovery rate of the method can meet the requirements of the standard method.Therefore,our laboratory has the ability to detect natamycin.

关 键 词：纳他霉素 外标法 高效液相色谱仪 方法学验证 

分 类 号：TS213.23[轻工技术与工程—粮食、油脂及植物蛋白工程] TS255.44[轻工技术与工程—食品科学与工程] O657.72[理学—分析化学]