甲羟戊酸逆转氟伐他汀钠对舌鳞癌细胞的抑制作用  

Mevalonic acid reverses the antitumor effects of fluvastatin sodium on tongue squamous cell carcinoma cells

作　　者：钱叶梅 王卫红[1] 曾琳[1] 李静宜 唐文广 张哲 QIAN Yemei;WANG Weihong;ZENG Lin;LI Jingyi;TANG Wenguang;ZHANG Zhe(Department of Oral and Maxillofacial Surgery,Affiliated Stomatology Hospital of Kunming Medical University,Oral Diseases Key Laboratory of Yunnan Province,650106,China)

机构地区：[1]昆明医科大学附属口腔医院口腔颌面外科,云南省口腔医学重点实验室,650106

出　　处：《实用口腔医学杂志》2024年第2期227-232,共6页Journal of Practical Stomatology

基　　金：云南省卫生科技计划项目(编号:2018NS212);云南省卫健委医学领军人才项目(编号:L-2018010);昆明医科大学研究生创新基金(编号:2019S096)。

摘　　要：目的:探究甲羟戊酸(MEV)及氟伐他汀钠(FS)对舌鳞癌细胞的抑制作用。方法:不同浓度的FS和MEV分别或联合处理舌鳞癌细胞HSC-4。用CCK-8、流式细胞术、划痕实验、免疫荧光和Western blot技术分别检测细胞增殖、凋亡、迁移以及RAS同源基因家族成员A(RHOA)、组织因子(TF)和BAX蛋白的表达情况。结果:FS抑制HSC-4细胞的增殖和迁移,降低细胞内RHOA和TF的表达,促进BAX表达。MEV逆转FS对HSC-4细胞的抑制作用,促进细胞内RHOA和TF的表达(P<0.05)。结论:MEV体外逆转FS对舌鳞癌细胞HSC-4的抑制作用。Objective:To investigate mevalonic acid(MEV)on reversing the antitumor effects of fluvastatin sodium(FS)on tongue squamous cell carcinoma(TSCC)cells.Methods:TSCC HSC-4 cells were treated with different concentrations of FS and MEV respectively or in combination.Cell counting kit-8,flow cytometry,wound healing,immunofluorescence and western blotting assays were conducted to examine cell proliferation,apoptosis and migration capacities,as well as the expression levels of Ras homolog family member A(RHOA),tissue factor(TF)and B-cell lymphoma-2-associated X(BAX)proteins,respectively.Results:FS inhibited HSC-4 cell proliferation and migration,decreased the expression of RHOA and TF,and increased BAX expression.MEV reversed the above antitumor effects of FS and promoted intracellular RHOA and TF expression.Conclusion:MEV reverses the antitumor effects of FS on HSC-4 cells in vitro.

关 键 词：舌鳞状细胞癌 氟伐他汀钠 甲羟戊酸 

分 类 号：R739.8[医药卫生—肿瘤]