Evaluating the tumor stratification with a lysosomal pH sensitive-probe by fluorescence lifetime imaging  

作　　者：Jun Yan Xing Liang Qian Zhang Luolin Wang Weiying Lin 

机构地区：[1]Guangxi Key Laboratory of Electrochemical Energy Materials,Institute of Optical Materials and Chemical Biology,School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China

出　　处：《Chinese Chemical Letters》2024年第3期262-267,共6页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21877048,22077048 and 22277014);Guangxi Natural Science Foundation(Nos.2021GXNSFDA075003,AD21220061);the Startup Fund of Guangxi University(No.A3040051003)。

摘　　要：Owing to the anaerobic metabolism in the tumor,abundant acidic metabolites are produced and accumulated in the cells.Therefore,the cells in different tumor layers are directly linked to the pH micro-environment.Nevertheless,due to the lack of robust tools,the high-efficient evaluation of the acidic micro-environment of tumor stratification faces the challenge of accurate diagnosis.We designed a new pH sensitive fluorescent lifetime probe target to lysosomes.As we expected,the fluorescence lifetime of PLN possesses a good linear fit to the pH value,which could detect the pH change at a single lysosome level in real time,and then evaluate the different acidity of tumor stratification.The probe PLN is successfully used to evaluate the tumor stratification by fluorescence lifetime imaging microscopy(FLIM)for the first time,which is of great significance in the preoperative diagnosis of clinical tumor treatment or evaluation of drug delivery effect.

关 键 词：FLIM pH Fluorescence imaging LYSOSOME Tumor imaging 

分 类 号：O657.3[理学—分析化学] R730.4[理学—化学]