羧甲基化柑橘果胶的体外模拟消化及益生元作用  

In vitro simulated digestion and intestinal prebiotic effect of carboxymethyl citrus pectin

作　　者：梁美香 杨可 李康杰 黎柏辉 姜铁民 李霞[2] LIANG Meixiang;YANG Ke;LI Kangjie;LI Bohui;JIANG Tiemin;LI Xia(Guilin University of Technology Hospital,Guilin 541000,China;College of Chemistry and Biology,Guilin University of Technology,Guilin 541000,China)

机构地区：[1]桂林理工大学医院,广西桂林541000 [2]桂林理工大学化学与生物工程学院,广西桂林541000

出　　处：《食品与发酵工业》2024年第7期173-179,共7页Food and Fermentation Industries

摘　　要：为探究羧甲基化柑橘果胶(carboxymethyl citrus pectin,CCP)在益生元方面的潜力,以NaOH-氯乙酸化学法制得CCP,采用红外光谱对其进行表征,模拟人体消化实验探究其抗消化性,并以其作碳源,研究其对短乳杆菌、植物乳杆菌、德氏乳杆菌保加利亚亚种和嗜热乳杆菌的益生元作用。结果表明,在红外光谱中1616、1419、1313 cm^(-1)处存在羧甲基的特征吸收峰,测定其取代度为0.4371,柑橘果胶羧甲基化修饰成功;在模拟人体抗消化实验中,CCP在模拟唾液、胃液和小肠液中的水解度分别低于3%、6%和2%,证明其能有效抵抗人体消化道消化;CCP促进短乳杆菌增殖的最佳质量浓度为15 g/L,对其他3种益生菌的最佳质量浓度为30 g/L,在最佳果胶质量浓度下,4种益生菌在18~30 h达到增殖的稳定期,细菌浓度达最高值,培养基pH值达到最低值,CCP对4种肠道益生菌的促进增殖作用均优于柑橘果胶。研究表明CCP是一种潜在的益生元,在医药保健等领域具有较高的发展潜力。To explore the potential of carboxymethyl citrus pectin(CCP)as a prebiotic,CCP was prepared by the chemical method of sodium hydroxide-chloroacetic acid,and the structure was characterized by infrared spectroscopy.Its anti-digestibility was explored by simulating the human digestive environment in this study,which used CCP as a carbon source to study its prebiotic effects on Lactobacillus brevis,Lactobacillus plantarum,Lactobacillus delbrueckii subsp.bulgaricus,and Streptococcus thermophilus.Results showed that CCP had characteristic absorption peaks at 1616,1419,and 1313 cm^(-1),which indicated that it was successfully modified and the degree of substitution was 0.4371.In the simulated human anti-digestion experiment,the hydrolysis degree of CCP in the simulated saliva,gastric juice,and intestinal juice was lower than 3%,6%,and 2%,respectively,indicating its resistance to digestion in the human digestive tract.The optimal mass concentration of CCP for promoting the proliferation of Lactobacillus brevis was 15 g/L,and for the other three probiotics was 30 g/L.Under the optimal mass concentration,the probiotics reached the stable period of proliferation in 18-30 h,the bacterial concentration reached the highest value,and the pH value of the medium reached the lowest value.The CCP was superior to citrus pectin in promoting the proliferation of four intestinal probiotics.CCP has the potential to be used as prebiotics and has high development potential in the fields of medicine and health care.

关 键 词：柑橘果胶 羧甲基化 模拟人体消化 益生元 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]