Qualitative and quantitative analysis ofβ-sitosterol marker in virgin camellia oil and virgin olive oil  

作　　者：李阳 吴旻谕 翟量 张辉 沈立荣 Yang Li;Minyu Wu;Liang Zhai;Hui Zhang;Lirong Shen(National Engineering Laboratory of Intelligent Food Technology and Equipment,Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture,Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture,Zhejiang Key Laboratory for Agro-Food Processing,Fuli Institute of Food Science,College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou,China;Innovation Center of Yangtze River Delta,Zhejiang University,Jiaxing,China)

机构地区：[1]National Engineering Laboratory of Intelligent Food Technology and Equipment,Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture,Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture,Zhejiang Key Laboratory for Agro-Food Processing,Fuli Institute of Food Science,College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou,China [2]Innovation Center of Yangtze River Delta,Zhejiang University,Jiaxing,China

出　　处：《Food Quality and Safety》2023年第4期579-587,共9页食品品质与安全研究（英文版）

基　　金：This research was funded by the“Pioneer”and“Leading Goose”R&D Program of Zhejiang Province(No.2023C02044);Zhejiang Province Key Research and Development Program(No.2021C02032);Zhejiang Provincial Natural Science Foundation(No.LQ20C200013),China.

摘　　要：Camellia oil and olive oil with superior economic value are easily adulterated with other,cheaper oils.It is difficult to identify both oils by traditional methods because of their similar fatty acid profiles.In the present study,a novel method for qualitative and quantitative analysis ofβ-sitosterol using GC/MS and GC/FID was developed to identify camellia oil and olive oil.The method validation ofβ-sitosterol analysis showed good linearity and satisfactory values for recovery,accuracy,precision,and repeatability.The linear regression coefficient(R^(2))of the calibration curve was 0.9985.An acceptable limit of detection(0.36 mg/100 g)and limit of quantification(1.20 mg/100 g)were achieved.The spiked recoveries were 95.0%to 100.3%.The relative standard deviation(RSD)of within-day precision was less than 3.26%,and the RSD of retention times and peak areas for repeatability were within 0.03%and 1.08%,respectively.The contents ofβ-sitosterol in virgin camellia oil and virgin olive oil were in the range of 14.1–30.2 mg/100 g and 94.3–173.2 mg/100 g,respectively,indicating that theβ-sitosterol content in the former is seven times that in the latter,andβ-sitosterol could be a potential marker for the authentication and adulteration detection of both oils.

关 键 词：Β-SITOSTEROL virgin camellia oil(VCO) virgin olive oil(VOO) qualitative analysis quantitative analysis 

分 类 号：TS225.17[轻工技术与工程—粮食、油脂及植物蛋白工程]