运动减肥改善肥胖小鼠卵子质量和早期胚胎发育的研究  

Exercise for weight loss improves oocyte viability and early embryonic development in obese mice

作　　者：喻婷 周雯 张茜 王怡[1] 鞠辉明[1] 李峰 YU Ting;ZHOU Wen;ZHANG Xi;WANG Yi;JU Huiming;LI Feng(Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonosis/College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Center for Reproductive Medicine,Jiangsu Subei People's Hospital/Yangzhou University School of Clinical Medicine,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院/江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [2]扬州大学临床医学院/江苏省苏北人民医院生殖医学中心,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2024年第1期93-100,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31872323);扬州市社会发展项目(SSF000150)。

摘　　要：为探讨肥胖对雌性动物生育力的影响并探索通过游泳减肥的运动方式改善肥胖对生殖影响的可行性,将3周龄C57BL/6小鼠分为3组:(1)高脂饮食建立肥胖小鼠模型组(HFD组);(2)肥胖小鼠通过运动(游泳锻炼)减肥组(SE组);(3)正常饮食饲养小鼠组(ND组)。通过统计卵巢系数、小鼠超数排卵后排卵数、卵母细胞体内及体外成熟率、体外受精后早期胚胎发育率、卵母细胞中纺锤体结构完整性、线粒体分布同质性来评估肥胖及游泳锻炼减肥对小鼠生育力的影响。结果表明:HFD组卵巢系数、超数排卵后排卵数、卵母细胞体外成熟率、卵巢中卵泡数量均显著低于ND和SE组,ND与SE组无显著差异,HFD组卵母细胞体外受精率和早期胚胎发育率均显著低于ND和SE组。HFD组小鼠MⅡ期卵母细胞纺锤体正常的比例[(22.94±4.73)%]显著低于ND[(85.63±6.63)%]和SE组[(81.05±5.5)%];HFD组小鼠均质型线粒体分布的百分率[(25.73±13.42)%]显著低于ND[(50.77±3.08)%]和SE组[(52.05±7.15)%]。这一研究提示肥胖对小鼠卵母细胞质量和早期胚胎发育均产生负面影响,通过游泳运动减肥可改善肥胖引起的负面影响,为后续深入研究肥胖对雌性动物生殖力影响提供了试验基础。In order to investigate the effect of obesity on the fertility of female animals and to explore the feasibility of improving the effect of obesity on reproduction through the exercise of swimming for weight loss,three-week-old C57BL/6 mice were divided into three groups:1) high-fat diet(HFD),2) sprot exercise to lose weight(SE),and 3) normal diet(ND).The effects of obesity on mouse fertility were assessed by counting the ovarian coefficient,the number of mice ovulating after supernumerary ovulation,the maturation rate of oocytes in vivo and in vitro,the rate of early embryonic development after in vitro fertilization,the structural integrity of spindle in oocytes,and the homogeneity of mitochondrial distribution,The results showed that the HFD group was significantly lower than the ND group and the SE group in these indexes of ovarian coefficient including number of supernumerary ovulations,in vitro maturation rate of eggs,in vitro maturation rate,and number of follicles in the ovaries(P<0.05),and there was no significant difference between the ND and SE groups(P>0.05).The spindle immunofluorescence results showed that the percentage of normal spindle in MII stage oocytes of mice in the HFD group [(22.94+4.73) %] was significantly lower than that in the ND group [(85.63±6.63)%] and the SE group [(81.05±5.5)%].Mitochondrial immunofluorescence results showed that the percentage of homogeneous mitochondrial distribution in mice in the HFD group [(25.73±13.42)%] was significantly lower than that in the ND group [(50.77±3.08)%] and the SE group [(52.05±7.15)%].The in vitro fertilization rate and early embryonic development rate of eggs in the HFD group were significantly lower than those in the ND and SE groups.These results indicate that obesity negatively affects oocyte quality and early embryonic development in mice,and that weight loss through swimming exercise can ameliorate the negative effects induced by obesity,and the research work provides an experimental basis for subsequent in-depth studies on the eff

关 键 词：肥胖 运动减肥 卵母细胞质量 胚胎发育 高脂饮食 

分 类 号：S814.1[农业科学—畜牧学]