Genome-wide identification and characterization of the PbrATG family in Pyrus bretschneideri and functional analysis of PbrATG1a in response to Botryosphaeria dothidea  

作　　者：Yun Wang Xun Sun Zhenwu Zhang Zhihua Xie Kaijie Qi Shaoling Zhang 

机构地区：[1]Center of Pear Engineering Technology Research,State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

出　　处：《Horticultural Plant Journal》2024年第2期327-340,共14页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No.31901989);Natural Science Foundation of Jiangsu Province (Grant No.BK20190534);China Postdoctoral Science Foundation (Grant No.2021T140332);Postgraduate Research&Practice Innovation Program of Jiangsu Province (Grant No.KYCX20_0584)。

摘　　要：The pear is an economic fruit that is widely planted around the world and is loved by people for its rich nutritional value. Autophagy is a self-protection mechanism in eukaryotes, and its occurrence often accompanied by the degradation of damaged substances in cells and the recycling of nutrients. Autophagy is one of the mechanisms through which plants respond to environmental stress and plays an important role in plant development and stress resistance. Functional studies of autophagy-related genes (ATGs) have been performed on a variety of plant species, but little information is available on the ATG family in pear (Pyrus bretschneideri Rehd). Therefore, we analyzed the evolutionary dynamics and performed a genome-wide characterization of the PbrATG gene family. A total of 28 PbrATG members were identified.Phylogenetic analysis showed that PbrATGs were more closely related to ATGs of European pear and apple. Evolutionary analysis revealed that whole-genome duplication (WGD) and dispersed duplication events were the main driving forces of PbrATG family expansion.Expression analysis of different pear tissues showed that all the genes were expressed in different pear tissues, and different PbrATGs are expressed at different times and in different locations. Moreover, all PbrATGs also responded to different abiotic stresses, especially salt and drought stress, which elicited the highest expression levels. Pear seedlings were subsequently infected with Botryosphaeria dothidea (B.dothidea). The results showed that different PbrATGs had different expression patterns at different infection stages. According to the gene expression data, PbrATG1a was selected as a key autophagy gene for further analysis. Silencing of PbrATG1a reduced the resistance of pear to B. dothidea, which resulted in increased lesions, reactive oxygen species (ROS) contents, antioxidant enzyme activity, and gene expression levels in the silenced pear seedlings after B. dothidea inoculation. In this study, a comprehensive bioinformatic analysis of

关 键 词：AUTOPHAGY PEAR PbrATGs Abiotic stress Botryosphaeria dothidea PbrATG1a 

分 类 号：S661.2[农业科学—果树学]