槟榔碱对人胃黏膜上皮细胞的毒性作用研究  

Toxic Effects of Arecoline on Human Stomach Mucosal Epithelial Cells

作　　者：李家旭 成钰莹 杨扬 王璧莹 蒋玉珍 陈建萍[2] 黎攀 杜冰[1] LI Jiaxu;CHENG Yuying;YANG Yang;WANG Biying;JIANG Yuzhen;CHEN Jianping;LI Pan;DU Bing(College of Food Science,South China Agriculture University,Guangzhou 510642,China;School of Chinese Medicine,University of Hong Kong,Hong Kong 999077,China)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]香港大学中医药学院,中国香港999077

出　　处：《食品工业科技》2024年第9期341-349,共9页Science and Technology of Food Industry

基　　金：广东省现代农业产业技术体系创新团队建设专项资金(2023KJ125)。

摘　　要：本文旨在探讨槟榔碱对人胃黏膜上皮细胞GES-1的毒性作用。用不同浓度(60、90、120、150μg/mL)的槟榔碱处理GES-1细胞,并检测GES-1细胞活力、活性氧水平(Reactive Oxygen Species,ROS)和线粒体膜电位(Mitochondrial Membrane Potential,MMP),测定GES-1细胞还原型谷胱甘肽(Glutathione,GSH)水平、超氧化物歧化酶(Superoxide Dismutase,SOD)活力、乳酸脱氢酶(Lactate dehydrogenase,LDH)活力、三磷酸腺苷(ATP)酶活力。测定肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白介素-6(Interleukin 6,IL-6)、血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)、转化生长因子-β(Transforming Growth Factor-β,TGF-β)水平。结果表明,与空白对照组相比,槟榔碱刺激后GES-1细胞形态发生改变,细胞活力最低降低至24%,活性氧水平最高升至12.5倍,线粒体膜电位最低下降至10.4%。在150μg/mL槟榔碱刺激下,总ATP酶以及Na^(+)K^(+)-ATP酶活力分别下降至42.31%和39.84%,GSH和SOD分别下降了40.23%和34.1%,LDH上升了30.46%,TNF-α、IL-6、VEGF、TGF-β水平分别上升了4.67%、10.2%、23.14%和22.83%。结果表明槟榔碱能够产生氧化应激并诱发炎症因子,对GES-1细胞造成毒性损伤。The purpose of this study was to look into the toxicity of arecoline on human stomach mucosal epithelial cells GES-1.GES-1 cells were treated with different concentrations(60,90,120,150μg/mL)of arecoline,detection of GES-1 cell viability,reactive oxygen species levels(ROS)and mitochondrial membrane potential(MMP).The levels of glutathione(GSH),superoxide dismutase(SOD),lactate dehydrogenase(LDH),adenosine triphosphate(ATP)enzymes were measured in GES-1 cellls.And the leves of tumor necrosis factor-α(TNF-α),interleukin 6(IL-6),vascular endothelial growth factor(VEGF),transforming growth factor-β(TGF-β)were measured in GES-1 cells.Compared with the control group,the shape of GES-1 cells was altered after arecoline stimulation,with the lowest cell viability rate reduced to 24%,the highest reactive oxygen species level increased to 12.5 fold,and the lowest mitochondrial membrane potential decreased to 10.4%.Under 150μg/mL arecoline stimulation,T-ATPase and Na^(+)K^(+)-ATPase activity decreased to 42.31% and 39.84%,GSH and SOD decreased by 40.23% and 34.1%,respectively,LDH increased by 30.46%,TNF-α,IL-6,VEGF and TGF-β levels increased by 4.67%,10.2%,23.14%and 22.83%.The results indicate that arecoline can produce oxidative stress and induce inflammatory factors that cause toxic damage to GES-1 cells.

关 键 词：槟榔碱 人胃黏膜上皮细胞GES-1 氧化应激 线粒体 细胞毒性 

分 类 号：TS201.4[轻工技术与工程—食品科学]