Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury  

作　　者：FEI KONG QIYUAN TIAN BINGLIN KUANG LILI SHANG XIAOXIAO ZHANG DONGYANG LI YING KONG 

机构地区：[1]Department of Endocrinology,Heilongjiang University of Chinese Medicine,Harbin,150040,China [2]Department of Cardiology,Heilongjiang University of Chinese Medicine,Harbin,150040,China [3]College of Clinical Medicine,Heilongjiang University of Chinese Medicine,Harbin,150040,China [4]Department of Nephrology,Beijing Hospital of Integrated Traditional Chinese and Western Medicine,Beijing,100039,China [5]College of Acupuncture and Massage,Heilongjiang University of Chinese Medicine,Harbin,150040,China [6]Department of Acupuncture,Heilongjiang University of Chinese Medicine,Harbin,150040,China

出　　处：《BIOCELL》2024年第4期665-675,共11页生物细胞（英文）

基　　金：This study was funded by the Joint Guidance Project of Heilongjiang Provincial Natural Science Foundation of China(LH2023H063);the Scientific Research Project of Academic Thought Inheritance of Chinese Medicine Great Master of Heilongjiang Provincial Administration of Traditional Chinese Medicine(ZHY2023-151).

摘　　要：Introduction:Myocardial ischemia-reperfusion(IR)injury has received widespread attention due to its damaging effects.Electroacupuncture(EA)pretreatment has preventive effects on myocardial IR injury.SLC26A4 is a Na+independent anion reverse transporter and has not been reported in myocardial IR injury.Objectives:Tofind potential genes that may be regulated by EA and explore the role of this gene in myocardial IR injury.Methods:RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA pretreatment.Myocardial infarction size was detected by TTC staining.Serum CK,creatinine kinase-myocardial band,Cardiac troponin I,and lactate dehydrogenase levels were determined by ELISA.The effect of SLC26A4 on cardiomyocyte apoptosis was explored by TUNEL staining and western blotting.The effects of SLC26A4 on inflammation were determined by HE staining,ELISA,and real-time PCR.The effect of SLC26A4 on the NF-κB pathway was determined by western blotting.Results:SLC26A4 was up-regulated in IR rats but downregulated in IR rats with EA pretreatment.Compared with IR rats,those with SLC26A4 knockdown exhibited improved cardiac function according to decreased myocardial infarction size,reduced serum LDH/CK/CK-MB/cTnI levels,and elevated left ventricular ejection fraction and fractional shortening.SLC26A4 silencing inhibited myocardial inflammation,cell apoptosis,phosphorylation,and nuclear translocation of NF-κB p65.Conclusion:SLC26A4 exhibited promoting effects on myocardial IR injury,while the SLC26A4 knockdown had an inhibitory effect on the NF-κB pathway.These results further unveil the role of SLC26A4 in IR injury.

关 键 词：Myocardial ischemia REPERFUSION SLC26A4 NF-κB pathway 

分 类 号：R542.2[医药卫生—心血管疾病]