Simultaneously quantifying hundreds of acylcarnitines in multiple biological matrices within ten minutes using ultrahigh-performance liquid-chromatography and tandem mass spectrometry  

作　　者：Jingxian Zhang Qinsheng Chen Lianglong Zhang Biru Shi Men Yu Qingxia Huang Huiru Tang 

机构地区：[1]State Key Laboratory of Genetic Engineering,School of Life Sciences,Human Phenome Institute,Zhangjiang Fudan International Innovation Center,Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics,Zhongshan Hospital,Fudan University,Shanghai,200032,China [2]Wuhan Laboratory for Shanghai Metabolome Institute(SMI)Ltd.,Wuhan,430000,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第1期140-148,共9页药物分析学报（英文版）

基　　金：financial supports from the National Key R&D Program of China(Grant Nos.:2022YFC3400700,2022YFA0806400,and 2020YFE0201600);Shanghai Municipal Science and Technology Major Project(Grant No.:2017SHZDZX01);the National Natural Science Foundation of China(Grant No.:31821002).

摘　　要：Acylcarnitines are metabolic intermediates of fatty acids and branched-chain amino acids having vital biofunctions and pathophysiological significances. Here, we developed a high-throughput method for quantifying hundreds of acylcarnitines in one run using ultrahigh performance liquid chromatography and tandem mass spectrometry (UPLC-MS/MS). This enabled simultaneous quantification of 1136 acylcarnitines (C0–C26) within 10-min with good sensitivity (limit of detection < 0.7 fmol), linearity (correlation coefficient > 0.992), accuracy (relative error < 20%), precision (coefficient of variation (CV), CV < 15%), stability (CV < 15%), and inter-technician consistency (CV < 20%, n = 6). We also established a quantitative structure-retention relationship (goodness of fit > 0.998) for predicting retention time (tR) of acylcarnitines with no standards and built a database of their multiple reaction monitoring parameters (tR, ion-pairs, and collision energy). Furthermore, we quantified 514 acylcarnitines in human plasma and urine, mouse kidney, liver, heart, lung, and muscle. This provides a rapid method for quantifying acylcarnitines in multiple biological matrices.

关 键 词：ACYLCARNITINE UPLC-MS/MS Quantitative structure-retention relationship Molecular phenotype 

分 类 号：R155.5[医药卫生—营养与食品卫生学]