吡咯喹啉醌合成及生产工艺研究进展  

Research progress in synthesis and production technology of pyrroloquinoline quinone

作　　者：刘东方 黄建忠[1] 陶勇[2] 杨欣伟 柯崇榕[1,2] LIU Dongfang;HUANG Jianzhong;TAO Yong;YANG Xinwei;KE Chongrong(Engineering Research Center of Industrial Microbiology of the Ministry of Education,National and Local Joint Engineering Research Center of Industrial Microbiology and Fermentation Technology,College of Life Sciences,Fujian Normal University,Fuzhou 350108,Fujian,China;CAS Key Laboratory of Microbial Physiological and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)

机构地区：[1]福建师范大学生命科学学院、工业微生物发酵技术国家地方联合工程研究中心、工业微生物教育部工程中心,福建福州350108 [2]中国科学院微生物研究所微生物生理与代谢工程重点实验室,北京100101

出　　处：《微生物学报》2024年第4期999-1018,共20页Acta Microbiologica Sinica

基　　金：国家自然科学基金(22377012,21807011);福建省自然科学基金面上项目(2021J01170,2021J01172)。

摘　　要：吡咯喹啉醌(pyrroloquinoline quinone,PQQ)是继烟酰胺和核黄素之后发现的第三类氧化还原酶辅因子,普遍存在于生物体中参与呼吸链电子传递,具有促进线粒体产生、清除自由基、增强细胞代谢和预防心肌损伤等生理功能,在医药、食品和农业领域具有广泛的应用前景。微生物发酵法是PQQ生产的主要方式,解析PQQ生物合成途径及其调控机制,通过代谢工程选育短周期、高产量的生产菌是PQQ工业化的研究方向之一。本文综述了PQQ的合成途径、高产菌株选育以及微生物发酵生产与分离纯化的研发工作,为深入阐释PQQ的生物合成机制和工业化生产菌株的选育提供参考。Pyrroloquinoline quinone(PQQ),the third oxidoreductase coenzyme discovered in the nature after nicotinamide and riboflavin,is ubiquitous in bacteria,fungi,plants,and animals.PQQ participates in a variety of life activities and has anti-inflammation,anti-oxidation,cell metabolism-enhancing,and cardioprotective activities,demonstrating broad application prospects in pharmaceuticals,agriculture,food and other fields.Therefore,the large-scale production of PQQ is the primary problem that needs to be solved at present.Microbial fermentation is a primary production method of PQQ.Deciphering the biosynthesis pathway and regulatory mechanism of PQQ is essential for the screening and breeding of strains with short production periods and high yields by metabolic engineering,which has been a hot topic in this field.This paper summarizes the synthesis pathways,strain screening and breeding,microbial production,and purification processes of PQQ,aiming to provide a reference for further research and application of PQQ.

关 键 词：吡咯喹啉醌 合成途径 菌株选育 代谢调控 分离纯化 

分 类 号：TQ25[化学工程—有机化工] TQ920.6[轻工技术与工程—发酵工程]