两株菌协同发酵鱼下脚料制备功能性宠物饲料  

Synergistically ferment fish scraps by two strains to prepare functional pet feed

作　　者：钟玉婷 朱佳雯 吴珊 刘莉菲 冯红丽 刘爱民[1] ZHONG Yu-ting;ZHU Jia-wen;WU Shan;LIU Li-fei;FENG Hong-li;LIU Ai-min(College of Life Sciences,Anhui Normal University,Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources,Wuhu 241000,China)

机构地区：[1]安徽师范大学生命科学学院//重要生物资源保护与利用研究安徽省重点实验室,安徽芜湖241000

出　　处：《粮食与饲料工业》2024年第2期68-71,75,共5页Cereal & Feed Industry

基　　金：国家级大学生创新创业训练计划(202210370160);2022年度安徽省高等学校省级质量工程重点项目(2022jyxm532)。

摘　　要：为了提高有机废弃物在生产中的利用,探索新型功能性宠物饲料,以鱼下脚料为原料,通过单因素试验和正交试验探究最佳发酵条件,并添加猴头菇菌丝体和番茄渣制成功能性饲料。结果表明,最优发酵条件为枯草芽孢杆菌∶红酵母=2∶1,鱼下脚料添加量30%、接菌量5%(V/V)、发酵温度30℃,发酵基质中添加烘干的2%猴头菇菌丝体和1%番茄渣,可显著增加原料中蛋白质和氨基酸含量,粗蛋白质含量提升率为73.71%,总氨基酸含量提升率为24.97%,其中,谷氨酸含量增加最为明显,提升率为98.98%。小鼠急性毒理试验表明,对照组与试验组无显著性差异(P>0.05),说明此饲料可应用于功能性宠物饲料的生产。该研究为今后鱼下脚料的发酵工艺提供了理论基础,且为宠物饲料的发展提供了新方向。In order to improve the utilization of organic waste in production,a new type of functional pet feed was explored,using fish scraps as raw materials,exploring the optimal fermentation conditions through single-factor test and orthogonal test,and adding Hericium erinaceus mycelium and tomato pomace to make functional feed.The results showed that the optimal fermentation conditions were Bacillus subtilis∶Rhodotorula=2∶1,30%of fish leftovers,5%of bacteria reception(v/v),fermentation temperature of 30℃,and 2%dried Hericium erinaceus mycelium and 1%tomato pomace were added to the fermentation matrix.It could significantly increase the content of protein and amino acids in raw materials,the crude protein improvement rate was 73.71%,the total amino acid improvement rate was 24.97%,among which,the glutamic acid content increases the most obviously,the increase rate is 98.98%.The acute toxicological test of mice showed that there was no significant difference between the control group and the experimental group(P>0.05),indicating that this feed could be applied to the production of functional pet feed.This study provided a theoretical basis for the fermentation process of fish scraps in the future,and provided a new direction for the development of pet feed.

关 键 词：微生物发酵 鱼下脚料 宠物饲料 粗蛋白质 

分 类 号：S816.6[农业科学—饲料科学] Q939.9[农业科学—畜牧学]