纳豆芽孢杆菌Bacillus natto NK4液态发酵产纳豆激酶的工艺优化  

Optimization of liquid fermentation condition of Bacillus natto NK4 for nattokinase production

作　　者：叶延欣 秦鹏 别鹏坤 张书斌 李蕾蕾 陈艳艳 张道雷[2,3] YE Yan-xin;QIN Peng;BIE Peng-kun;ZHANG Shu-bin;LI Lei-lei;CHEN Yan-yan;ZHANG Dao-lei(School of Life Sciences&Engineering,Henan University of Urban Construction,Pingdingshan 467036,China;School of Bioengineering,Shandong Polytechnic,Jinan 250000 China;Henan Provincial Key Laboratory of Water Pollution Control&Rehabilitation Technology,Henan University of Urban Construction,Pingdingshan 467036,China)

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036 [2]山东职业学院生物工程学院,山东济南250000 [3]河南省水体污染防治与修复重点实验室,河南平顶山467036

出　　处：《河南城建学院学报》2024年第2期103-108,132,共7页Journal of Henan University of Urban Construction

基　　金：河南城建学院博士科研启动项目(Q2018005);河南省水体污染防治与修复重点实验室开放基金项目(CJSP2022004)。

摘　　要：为提高纳豆激酶的产量,采用单因素试验及正交试验对纳豆芽孢杆菌Bacillus natto NK4进行发酵条件优化,获得该菌种液体发酵产酶的最优条件。结果表明,纳豆芽孢杆菌产纳豆激酶最佳的发酵条件为接种量2%,培养基初始pH值6.0,培养温度34℃,发酵时间72 h。此时,纳豆激酶酶活力由848.28 IU/mL提高到1569.31 IU/mL。To improve the production of nattokinase,single-factor experiments and orthogonal experiments were conducted to optimize the fermentation conditions of Bacillus natto NK4.The optimal conditions for liquid fermentation of this strain were obtained.The results showed that the optimal fermentation conditions for Bacillus natto to produce nattokinase were an inoculum size of 2%,an initial pH of 6.0,a fermentation temperature of 34℃,and a fermentation time of 72.0 h.Under these conditions,the enzyme activity of nattokinase increased from 848.28 IU/mL to 1569.31 IU/mL.

关 键 词：Bacillus natto NK4 纳豆激酶 正交试验 发酵条件 

分 类 号：Q93[生物学—微生物学]