超声波联合木瓜蛋白酶降解壳聚糖的工艺研究  

Study on the process of chitosan degradation by ultrasonic combined with papain

作　　者：杨庆红 黄永春[1,2] 张昆明[1,2] YANG Qinghong;HUANG Yongchun;ZHANG Kunming(School of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Guangxi Key Laboratory of Green Processing of Sugar Resources(Guangxi University of Science and Technology),Liuzhou 545006,China)

机构地区：[1]广西科技大学生物与化学工程学院,广西柳州545006 [2]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006

出　　处：《广西科技大学学报》2024年第2期97-102,共6页Journal of Guangxi University of Science and Technology

基　　金：国家自然科学基金项目(31660472)资助。

摘　　要：采用超声波技术联合木瓜蛋白酶对壳聚糖进行降解,以还原糖释放量为指标,通过单因素实验研究了壳聚糖质量浓度、溶液p H、超声功率及超声时间对壳聚糖降解的影响。并利用傅里叶变换红外光谱(FT-IR)及X-射线衍射(XRD)分别对降解前后的壳聚糖进行结构表征分析。结果表明:超声波联合木瓜蛋白酶可以有效降解壳聚糖,在壳聚糖质量浓度为6 g/L、溶液p H为4.5、超声功率为480 W、超声时间为180 min条件下,加入木瓜蛋白酶,降解效果最明显,还原糖释放量达到1.456 g/L;FT-IR结果表明,降解后壳聚糖的结构基本没有变化;XRD结果表明,降解后壳聚糖的晶体结构受到了破坏。Chitosan was degraded by ultrasonic technology combined with papain.Taking the release of reducing sugars as an index,the effects of chitosan concentration,solution pH,ultrasonic power and ultrasonic time on chitosan degradation were investigated by univariate experiments.Fourier transform infrared spectroscopy(FT-IR)and X-ray diffraction(XRD)were used to characterize the structure of chitosan before and after degradation.The results showed that ultrasonic combined with papain could effectively degrade chitosan.Under the condition of chitosan concentration of 6 g/L,the solution pH of 4.5,ultrasonic power of 480 W,ultrasonic time of 180 min,papain was added to continue the reaction,and the degradation effect was the most obvious,and the release of reducing sugars reached 1.456 g/L.The FT-IR results showed that the structure of chitosan remained basically unchanged after degradation.XRD results show that the crystal structure of chitosan was destroyed after degradation.

关 键 词：壳聚糖 超声波 木瓜蛋白酶 降解工艺 

分 类 号：Q63[生物学—生物物理学]