新型冠状病毒阳性与阴性患者流产组织染色体对比分析  

Comparative chromosomal analysis of miscarried tissues from new crown positive and negative patients

作　　者：张晨 杨惠云 刘小雪 邵小光 石杨 张静[3] 朱文秀 石铭 Zhang Chen;Yang Huiyun;Liu Xiaoxue;Shao Xiaoguang;Shi Yang;Zhang Jing;Zhu Wenxiu;Shi Ming(Dalian Medical University College of Laboratory Medicine,Dalian 116044,China;Department of Laboratory,Dalian Municipal Women and Children′s Medical Center(Group),Dalian 116037,China;Department of Laboratory,Dalian Municipal Center Hospital,Dalian 116033,China)

机构地区：[1]大连医科大学检验医学院,大连116044 [2]大连市妇女儿童医疗中心(集团)体育新城院区检验科,大连116037 [3]大连市中心医院检验科,大连116033

出　　处：《中国医师进修杂志》2024年第4期317-321,共5页Chinese Journal of Postgraduates of Medicine

基　　金：大连市临床重点专科登峰计划;大连市妇女儿童医疗中心(集团)博士启动基金项目([2022]156)。

摘　　要：目的采用低深度全基因组染色体拷贝数变异测序(CNV-seq)技术探究新型冠状病毒(简称新冠)阳性和阴性患者对自然流产组织染色体异常的影响。方法回顾性选取2022年6月至2023年2月就诊于大连市妇女儿童医疗中心(集团)的146例自然流产患者(66例新冠阳性患者和80例新冠阴性患者),按照其新冠检测结果、年龄、流产次数和怀孕方式进行分组并分析各组染色体情况。结果146例流产组织中异常染色体核型97例,占66.44%,其中数目异常50例,结构异常34例,嵌合体6例,其他异常类型7例。新冠阴性组染色体异常率高于新冠阳性组[72.50%(58/80)比59.09%(39/66)],差异无统计学意义(P>0.05);年龄≥35岁组染色体异常率高于<35岁组[71.88%(46/64)比62.19%(51/82)],差异无统计学意义(P>0.05);流产次数≥2次组染色体异常率高于首次流产组[67.61%(48/71)比65.33%(49/75)],差异无统计学意义(P>0.05);辅助生殖助孕组染色体异常率高于自然受孕组[68.89%(31/45)>65.35%(66/101)],差异无统计学意义(P>0.05)。97例异常染色体核型结果中,新冠阳性患者三体型发生率高于新冠阴性组[66.67%(26/39)比17.24%(10/58)],差异有统计学意义(P<0.01)。结论流产组织染色体异常率在新冠阳性和阴性患者间差异无统计学意义,但异常核型中三体型发生率在新冠阳性和阴性患者组间差异有统计学意义。Objective To investigate the effect of chromosomal abnormalities on spontaneous abortion tissues in COVID-19 positive and negative patients using low depth whole genome chromosome copy number variation sequencing(CNV-seq)technique.Methods One hundred and forty-six patients with spontaneous abortion(66 new crown positive patients and 80 new crown negative patients)attending Dalian Women and Children′s Medical Center(Group)from June 2022 to February 2023 were selected and grouped according to their new crown test results,age,number of abortions and mode of pregnancy and analyzed for chromosomal status in each group.Results There were 97 cases of abnormal karyotype in 146 aborted tissues,accounting for 66.44%,including 50 cases of numerical abnormalities,34 cases of structural abnormalities,6 cases of chimerism,and 7 cases of other abnormal types.The rate of chromosomal abnormalities was higher in the new crown negative group than in the new crown positive group:72.50%(58/80)vs.59.09%(39/66),with no statistically significant(P>0.05);the rate of chromosomal abnormalities in the age≥35 years group was higher than that in the<35 years group:71.88%(46/64)vs.62.19%(51/82),with no statistically significant difference(P>0.05);the rate of chromosomal abnormalities in the group with≥2 miscarriages was higher than that in the group with the first miscarriage:67.61%(48/71)vs.65.33%(49/75),with no statistically significant(P>0.05);the rate of chromosomal abnormalities in the group of assisted reproduction for fertilization was higher than that in the group of natural conception:68.89%(31/45)vs.65.35%(66/101),with no statistically significant(P>0.05).Of the 97 cases with abnormal karyotype results,the incidence of trisomy 21 was higher in the neo-crown-positive patients than in the neo-crown-negative group:66.67%(26/39)vs.17.24%(10/58),P<0.01.Conclusions There is no significant difference in the rate of chromosomal abnormalities in miscarried tissues between the new crown positive and negative patients,but the incidence of

关 键 词：冠状病毒 性染色体异常 自然流产 基因测序 

分 类 号：R714.21[医药卫生—妇产科学] R511[医药卫生—临床医学]