酿酒葡萄“赤霞珠”早熟株系的酚类物质差异研究  

Study on the Difference of Phenolic Compounds in Wine Grape“Cabernet Sauvignon”

作　　者：刘思凡 刘子雨 杨婷 单守明[4] LIU Si-fan;LIU Zi-yu;YANG Ting(Ningxia Hui Autonomous Region Agricultural Exploration and Design Institute,Yinchuan,Ningxia 750021;The Management Committee of the East Foothills of Ningxia Helan Mountains,Yinchuan,Ningxia 750021;Ningxia Vocational and Technical College of Wine and Desertification Control,Yinchuan,Ningxia 750021)

机构地区：[1]宁夏回族自治区农业勘查设计院,宁夏银川750021 [2]宁夏贺兰山东麓葡萄酒产业园区管理委员会,宁夏银川750021 [3]宁夏葡萄酒与防沙治沙职业技术学院,宁夏银川750021 [4]宁夏大学农学院,宁夏银川750021

出　　处：《安徽农业科学》2024年第8期169-172,176,共5页Journal of Anhui Agricultural Sciences

基　　金：宁夏回族自治区农业育种专项(NXNYYZ20210102)。

摘　　要：以欧亚种红色酿酒葡萄“赤霞珠”的4种不同成熟株系(提前9 d转色株系E1、提前7 d转色株系E2、提前5 d转色株系E3、正常转色株系CK)果实为材料,采用UPLC法测定果实中表儿茶素没食子酸酯、丁香酸、柚皮苷等22种单体酚含量,探索不同成熟株系间葡萄果实中单体酚积累的差异。结果表明:与正常成熟株系(CK)相比,提前5 d转色的成熟株系(E3)总单宁和总花色苷含量最高,分别为1.20和3.27 mg/g;9种酚酸类物质中2,5-二羟基苯甲酸含量在3个早熟株系中占比均为最高,且在E2和E3中占比均超过40%;6种黄烷醇类物质中表没食子儿茶素没食子酸酯含量在E1、E2、E3这3个早熟株系中的占比均为最高,分别占黄烷醇类物质总量的46%、57%、67%;7种黄酮醇类物质中杨梅素在早熟株系(E1、E2、E3)和正常成熟株系(CK)中的含量均为最高,分别占黄酮醇类物质总量的87%、93%、92%、89%;随着成熟时间的不断延后,3种酚类物质均呈现先上升后下降的趋势,在早熟株系中,黄酮醇类物质含量均高于酚酸类和黄烷醇类物质,黄烷醇类物质“赤霞珠”葡萄中积累量较低。Using the fruits of four different mature strains of the Eurasian red wine grape“Cabernet Sauvignon”(transgenic line E19 days in advance,transgenic line E27 days in advance,transgenic line E35 days in advance,and normal transgenic line CK)as materials,the UPLC method was used to determine the content of 22 monomeric phenols such as epigallocatechin gallate,syringic acid and naringin in fruits,and to explore the differences of monomer phenol accumulation in grape fruits among different mature strains.The results showed that compared with normal mature lines(CK),the contents of total tannins and total anthocyanins in mature lines(E3)changed 5 days earlier were the highest,which were 1.20 and 3.27 mg/g,respectively.The content of 2,5-dihydroxybenzoic acid in 9 phenolic acids was the highest in the three early maturing lines,and accounted for more than 40%in E2 and E3.The content of epigallocatechin gallate among the six flavanols was the highest in the three early maturity strains(E1,E2,E3),accounting for 46%,57%and 67%of the total flavanols,respectively.The content of myricetin in the seven flavonols was the highest in the early maturing(E1,E2,E3)and normal mature strains(CK),accounting for 87%,93%,92%and 89%of the total flavonols,respectively.As the ripening time continued to delay,the three phenolic substances all showed a trend of increasing first and then decreasing.In the early maturing strains,the flavonols content was higher than that of phenolic acids and flavanols,and the accumulation of flavanols in“Cabernet Sauvignon”grapes was low.

关 键 词：酿酒葡萄 早熟株系 酚类物质 积累量 

分 类 号：S663.1[农业科学—果树学]