基于随机蛙跳筛选的初烤烟叶中β-胡萝卜素和叶黄素含量近红外模型的建立  

Establishment of near infrared models of flue-cured tobacco leavesβ-carotene and lutein based on random frog screening

作　　者：何屹 杨本刚 尹嵩 陈实 尹晓东 周文忠 贾学伟 杨盼盼 HE Yi;YANG Bengang;YIN Song;CHEN Shi;YIN Xiaodong;ZHOU Wenzhong;JIA Xuewei;YANG Panpan(Sichuan Sanlian New Materials Co.,Ltd.,Chengdu 610101,China;Bijie Cigarette Factory,Guizhou Zhongyan Industrial Co.,Ltd.,Bijie 551799,China;Yunnan Tobacco Leaf Company,Kunming 652017,China;Yunnan Tongchuang Testing Technology Co.,Ltd.,Kunming 650106,China;College of Tobacco Science and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,China)

机构地区：[1]四川三联新材料有限公司,四川成都610101 [2]贵州中烟工业有限责任公司毕节卷烟厂,贵州毕节551799 [3]云南省烟草烟叶公司,云南昆明652017 [4]云南同创检测技术股份有限公司,云南昆明650106 [5]郑州轻工业大学烟草科学与工程学院,河南郑州450001

出　　处：《轻工学报》2024年第2期100-106,共7页Journal of Light Industry

基　　金：四川省重点研发项目(2018GZ0546);中国烟草总公司项目(2021530000242045)。

摘　　要：为提高近红外光谱法测定初烤烟叶中β-胡萝卜素和叶黄素含量的准确性和稳定性,采用随机蛙跳(Random Frog,RF)筛选特征波长建立上述指标的偏最小二乘(Partial Least Squares,PLS)近红外模型,结合模型内部交互验证结果确定最优建模波长数和主成分数,并对最优模型的准确性和稳定性进行内外部评价。结果表明:相较于全波长(1298个波长)建模,利用RF筛选出的181个和216个特征波长建模,模型的决定系数(R2)由0.7718和0.8677分别提高至0.9511和0.9587,校正均方根误差(RMSEC)由3.362μg/g和4.930μg/g分别降低至1.556μg/g和2.756μg/g,交互验证均方根误差(RMSECV)由3.304μg/g和4.836μg/g分别降低至1.529μg/g和2.703μg/g;模型外部预测集(样品数=50)的预测平均相对误差分别由11.80%和9.62%降低至5.48%和5.49%。采用RF筛选特征波长能有效提升初烤烟叶β-胡萝卜素和叶黄素含量近红外模型的准确性和稳定性,可为初烤烟叶质量评价提供理论参考。To improve the accuracy and stability of rapid determination ofβ-carotene and lutein in flue-cured by near infrared spectroscopy,this study used random frog to screen the characteristic wavelength of partial least squares(PLS)near infrared model of the above indicators.Furthermore,the optimal number of wavelengths and principal components were determined by internal interaction verification,and the accuracy and stability of the optimal model were verified by internal parameters and external parameters.The results showed that:compared with the full-wavelength(1298 wavelengths)model,the determination coefficient R2 of theβ-carotene and lutein models established 181 and 216 wavelength variables by RF wavelength screening combined with PLS was increased from 0.7718 and 0.8677 to 0.9511 and 0.9587,respectively.The corrected values of root mean square error of calibration(RMSEC)was reduced from 3.362μg/g and 4.930μg/g to 1.556μg/g and 2.756μg/g,respectively.The values of root mean square error of cross validation(RMSECV)was reduced from 3.304μg/g,4.836μg/g to 1.529μg/g,2.703μg/g;The average relative error of external validation(50 samples)decreased from 11.80%and 9.62%to 5.48%and 5.49%,respectively.The random frog screening wavelength method could significantly improve the accuracy and stability of the near infrared models for the determination ofβ-carotene and lutein of fluecured tobacco,which could provide more accurate and reliable data reference for its quality evaluation.

关 键 词：近红外模型 随机蛙跳 初烤烟叶 Β-胡萝卜素 叶黄素 

分 类 号：TS41[农业科学—烟草工业]