Experimental measurement and numerical modeling of deformation behavior of breast cancer cells passing through constricted microfluidic channels  

作　　者：Pouyan Keshavarz Motamed Hesam Abouali Mahla Poudineh Nima Maftoon 

机构地区：[1]Department of Systems Design Engineering,University of Waterloo,Waterloo,ON N2L 3G1,Canada [2]Center for Bioengineering and Biotechnology,University of Waterloo,Waterloo,ON N2L 3G1,Canada [3]Department of Electrical and Computer Engineering,University of Waterloo,Waterloo,ON N2L 3G1,Canada

出　　处：《Microsystems & Nanoengineering》2024年第1期129-147,共19页微系统与纳米工程（英文）

基　　金：supported by the Natural Sciences and Engineering Research Council of Canada(RGPIN-2020-05522&RGPIN-2020-00428).

摘　　要：During the multistep process of metastasis,cancer cells encounter various mechanical forces which make them deform drastically.Developing accurate in-silico models,capable of simulating the interactions between the mechanical forces and highly deformable cancer cells,can pave the way for the development of novel diagnostic and predictive methods for metastatic progression.Spring-network models of cancer cell,empowered by our recently proposed identification approach,promises a versatile numerical tool for developing experimentally validated models that can simulate complex interactions at cellular scale.Using this numerical tool,we presented spring-network models of breast cancer cells that can accurately replicate the experimental data of deformation behavior of the cells flowing in a fluidic domain and passing narrow constrictions comparable to microcapillary.First,using high-speed imaging,we experimentally studied the deformability of breast cancer cell lines with varying metastatic potential(MCF-7(less invasive),SKBR-3(medium-high invasive),and MDA-MB-231(highly invasive)in terms of their entry time to a constricted microfluidic channel.We observed that MDA-MB-231,that has the highest metastatic potential,is the most deformable cell among the three.Then,by focusing on this cell line,experimental measurements were expanded to two more constricted microchannel dimensions.The experimental deformability data in three constricted microchannel sizes for various cell sizes,enabled accurate identification of the unknown parameters of the spring-network model of the breast cancer cell line(MDA-MB-231).Our results show that the identifed parameters depend on the cell size,suggesting the need for a systematic procedure for identifying the size-dependent parameters of spring-network models of cells.As the numerical results show,the presented cell models can simulate the entry process of the cell into constricted channels with very good agreements with the measured experimental data.

关 键 词：STRICT PASSING NUMERICAL 

分 类 号：R730[医药卫生—肿瘤]