甲基莲心碱对避水应激大鼠结肠运动的影响及其机制  

The effect of Neferine on colonic motility induced by water avoidance stress in rats and its mechanism

作　　者：夏源 罗和生[1] XIA Yuan;LUO Hesheng(Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《胃肠病学和肝病学杂志》2024年第4期361-364,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究甲基莲心碱对避水应激大鼠结肠运动的影响和相关机制。方法建立避水应激大鼠模型,记录造模期间每组大鼠每日平均粪粒数,通过HE染色观察大鼠结肠病理改变。通过RM6240多通道生理信号系统记录造模大鼠体外结肠运动,以及不同浓度的甲基莲心碱对正常大鼠离体结肠肌条收缩活动的影响,通过Western blotting评估大鼠结肠神经元一氧化氮合成酶蛋白(neuronal nitric oxide synthase,nNOS)的表达水平。结果甲基莲心碱明显抑制避水应激所导致的大鼠结肠运动亢进,同时在体外水平以浓度依赖性的方式降低环形肌条和纵行肌条的收缩幅度。Western blotting结果显示,与对照组相比,给药组大鼠的nNOS蛋白表达上调。结论甲基莲心碱能抑制避水应激导致的大鼠结肠运动亢进,并且这种作用可能是通过一氧化氮途径实现的。Objective To preliminarily investigate the effects and potential mechanisms of Neferine on colonic motility induced by water avoidance stress in rats.Methods A rat model of water avoidance stress was established,and the average daily number of fecal pellets per day in each group during the modeling period was recorded.Pathological changes in the colon of rats were observed by HE staining.The RM6240 multi-channel physiological signaling device was used to monitor in vitro colonic motility in model rats and to examine the impact of various Neferine concentrations on the contractile activity of isolated colonic muscle strips in normal rats.The expression of neuronal nitric oxide synthase(nNOS)in the rat colon was quantified by Western blotting.Results Neferine decreased the contraction amplitude of circular and longitudinal muscle strips in a concentration-dependent manner in vitro,significantly inhibiting the hypermotility of the colon in rats induced by water avoidance stress.According to Western blotting data,nNOS protein expression was elevated in the treated rats compared to the control group.Conclusion Neferine reverses colonic motility hyperactivity in water avoidance stress rats,and this action may be partially mediated by the nitric oxide(NO)pathway.

关 键 词：甲基莲心碱 避水应激 NNOS 结肠高动力 

分 类 号：R576[医药卫生—消化系统]