聚集诱导发光微球免疫层析试纸条定量检测水产品中孔雀石绿  

Aggregation-induced luminescent microsphere based immunochromatographic assay for the quantitative detection of malachite green in aquatic products

作　　者：罗凯 张威[1] 刘文娟 郭平 熊勇华 LUO Kai;ZHANG Wei;LIU Wen-Juan;GUO Ping;XIONG Yong-Hua(Food Inspection and Testing Research Institute of Jiangxi General Institute of Testing and Certification,Nanchang 330052,China;Jiangxi-OAI Joint Research Institute,Nanchang University,Nanchang 330047,China;Jiangxi Weibang Biotechnology Co.,Ltd.,Nanchang 330096,China)

机构地区：[1]江西省检验检测认证总院食品检验检测研究院,南昌330052 [2]南昌大学中德联合研究院,南昌330047 [3]江西省维邦生物科技有限公司,南昌330096

出　　处：《食品安全质量检测学报》2024年第6期83-92,共10页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(32160599);江西省重点研发计划项目(20232BBG70030)。

摘　　要：目的建立一种用于定量检测水产品中孔雀石绿的高灵敏免疫层析检测新方法。方法以聚集诱导发光荧光微球为标记探针,制备荧光免疫层析试纸条,通过T/C比值法构建定量检测孔雀石绿的定量标准曲线,并实现水产品中孔雀石绿的高灵敏、定量检测。结果本方法定量检测水产品中孔雀石绿具有较高的检测灵敏度,其50%抑制率达到0.17μg/kg,最低检出限为0.05μg/kg。样本加标0.25、0.50、1.00μg/kg的平均回收率介于116.01%~122.76%,变异系数介于8.66%~11.71%(n=10),表明所建立的荧光免疫层析方法定量检测水产品中孔雀石绿具有较好的准确性、精密度。通过检测30份孔雀石绿阴性以及12份阳性的水产样本,结果表明所建立的荧光免疫层析方法无假阳性,且检测孔雀石绿含量与液相色谱-串联质谱法具有良好的一致性(线性相关系数为0.9695)。结论本研究以聚集诱导发光荧光微球为新型标记探针,建立了一种高灵敏检测水产品中孔雀石绿的定量方法,为水产品中孔雀石绿的筛查检测提供了技术支撑。Objective To establish a new highly sensitive immunochromatographic assay for quantitative detection of malachite green in aquatic products.Methods Fluorescent immunochromatographic test strips were prepared by using aggregation-induced luminescence fluorescent microspheres as labeled probes,and the quantitative standard curve for quantitative detection of malachite green was constructed by T/C ratio method,and the highly sensitive and quantitative detection of malachite green in aquatic products was realized.Results The quantitative detection of malachite green in aquatic products by this method had high detection sensitivity,its 50%inhibition rate reaches 0.17μg/kg,and the minimum limit of detection was 0.05μg/kg.The average recoveries of 0.25,0.50 and 1.00μg/kg ranged from 116.01%to 122.76%,and the coefficient of variation ranged from 8.66%to 11.71%(n=10),indicating that the established fluorescence immunochromatographic assay for quantitative detection of malachite green in aquatic products had good accuracy and precision.By detecting 30 samples of aquatic products with malachite green negative and 12 samples with malachite green positive,the results showed that the established fluorescence immunochromatographic assay had no false positive,and the detection of malachite green content had good consistency with liquid chromatography-tandem mass spectrometry(linear correlation coefficient was 0.9695).Conclusion In this study,a highly sensitive quantitative method for the detection of malachite green in aquatic products is established by using aggregation-induced luminescence fluorescent microspheres as new labeling probes,which provide technical support for the screening and detection of malachite green in aquatic products.

关 键 词：聚集诱导发光荧光微球 孔雀石绿 免疫层析方法 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]