猕猴桃果实后熟软化过程中己糖激酶基因的表达分析  

Expression Analysis of Hexokinase Genes in Kiwifruit during Postharvest Softening

作　　者：董文娟 陈明 向妙莲 陈金印 曾教科 DONG Wenjuan;CHEN Ming;XIANG Miaolian;CHEN Jinyin;ZENG Jiaoke(Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits&Vegetables,College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学农学院,江西省果蔬保鲜与无损检测重点实验室,江西南昌330045

出　　处：《食品科学》2024年第9期189-196,共8页Food Science

基　　金：江西省“双千计划”项目(jxsq2020101076);江西农业大学科研启动基金项目(9232309106)。

摘　　要：为解决猕猴桃果实采后快速软化问题,本研究以‘红阳’猕猴桃果实为材料,探究10 mmol/L N-乙酰-D-葡萄糖胺(N-acetyl-D-glucosamine,NAG)对猕猴桃后熟软化过程中基本生理变化、己糖激酶(hexokinase,HXK)活性和AcHXKs基因表达的影响。结果表明,与对照组(CK组)相比,外源NAG处理可有效降低猕猴桃的呼吸速率和乙烯释放量,延缓其硬度和总酸含量的下降以及果胶和淀粉的水解,维持较低的可溶性固形物含量。与CK组相比,NAG能够同时抑制HXK活性和AcHXK3表达水平,进而影响糖代谢和呼吸代谢过程。此外,AcHXK7~AcHXK9基因被NAG诱导上调表达,且表达量与硬度显著正相关,其可能作为糖信号分子介导猕猴桃后熟软化进程。综上,AcHXK3、AcHXK7~AcHXK9受NAG诱导差异表达,进而发挥催化和调节功能,延缓猕猴桃后熟软化。本实验结果可为猕猴桃后熟软化机制提供理论依据,也为猕猴桃保鲜技术的研发提供新思路。To solve the problem of the rapid softening of postharvest kiwifruit,the effect of 10 mmol/L N-acetyl-Dglucosamine(NAG)treatment on physiological changes,hexokinase(HXK)activity and AcHXKs gene expression in‘Hongyang’kiwifruit during postharvest ripening.The results showed that compared with the control group,exogenous NAG treatment could effectively inhibit the respiration rate and ethylene production of kiwifruit,delay the decline of firmness and total acid content,as well as the hydrolysis of starch and pectin,thereby maintaining a lower soluble solid content.In addition,NAG inhibited HXK activity and AcHXK3 expression level,thus affecting sugar and respiratory metabolism.Moreover,the expression of AcHXK7,8 and 9 was upregulated,which was positively correlated with firmness and probably involved in glucose sensor-mediated postharvest softening of kiwifruit.In conclusion,NAG treatment can induce the differential expression of AcHXK3,7,8 and 9,thereby delaying kiwifruit postharvest ripening via catalytic and regulatory functions.These results may provide a theoretical basis for the mechanism of kiwifruit postharvest softening,and also help researchers develop novel strategies for kiwifruit preservation.

关 键 词：猕猴桃 N-乙酰-D-葡萄糖胺 后熟软化 己糖激酶 

分 类 号：S663.4[农业科学—果树学]