用于分离单糖与寡糖的亲水色谱材料制备及应用进展  

Research Progress in the Preparation and Applications of Hydrophilic Chromatographic Materials for Separation of Monosaccharides and Oligosaccharides

作　　者：张丹丹 矫芮文 米春孝 陈菊 李想 汪秋宽 任丹丹 何云海 武龙 周慧 ZHANG Dandan;JIAO Ruiwen;MI Chunxiao;CHEN Ju;LI Xiang;WANG Qiukuan;REN Dandan;HE Yunhai;WU Long;ZHOU Hui(College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China;National R&D Branch Center for Seaweed Processing,Dalian 116023,China;Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province,Dalian 116023,China)

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁大连116023 [2]国家海藻加工技术研发分中心,辽宁大连116023 [3]辽宁水产品加工及综合利用重点实验室,辽宁大连116023

出　　处：《食品科学》2024年第9期261-271,共11页Food Science

基　　金：国家自然科学基金青年科学基金项目(32101861)。

摘　　要：糖类化合物在食品科学、医药科学、生命科学等领域中发挥至关重要的作用。糖类化合物可分为单糖、寡糖和多糖,其中单糖和寡糖大多是可溶性糖,不仅可以充当结构物质,还具有抗氧化、抗癌、抗菌、抗病毒等多种生物活性。亲水作用色谱弥补了反相液相色谱在糖类化合物分离方面的不足。本文重点介绍了2017—2022年报道的适合分离分析单糖和寡糖的氨基键合固定相、酰胺类键合固定相、糖类键合固定相、两性离子键合固定相等亲水色谱固定相,并概述了乙腈含量、缓冲盐浓度、流动相pH值和柱温等色谱分离条件对亲水分离效果的影响,以期为单糖及寡糖的分离分析提供一定的参考。Carbohydrates play a vital role in food science,medicine science,life science and other fields.Carbohydrates can be divided into monosaccharides,oligosaccharides,and polysaccharides,among which monosaccharides and oligosaccharides are mostly soluble,which can not only serve as structural substances,but also have various biological activities such as antioxidant,anticancer,antibacterial,and antiviral activities.Hydrophilic interaction chromatography makes up for the deficiency of reversed-phase liquid chromatography in the separation of carbohydrates.This paper reviews the stationary phases including amino bonded stationary phase,amide bonded stationary phase,carbohydrate bonded stationary phase and zwitterionic bonded stationary phase used in hydrophilic interaction chromatography for the separation and analysis of monosaccharides and oligosaccharides during 2017–2022,and summarizes the effects of chromatographic separation conditions such as acetonitrile content,buffer salt concentration,mobile phase pH and column temperature on the hydrophilic separation efficiency so as to provide a reference for the separation and analysis of monosaccharides and oligosaccharides.

关 键 词：单糖 寡糖 分离 色谱固定相 亲水作用色谱 

分 类 号：TS203[轻工技术与工程—食品科学]