Ethyl acetate fraction of Sargassum pallidum extract attenuates particulate matter-induced oxidative stress and inflammation in keratinocytes and zebrafish  

作　　者：Wook Chul Kim Ji-Won Park Bohyun Yun WonWoo Lee Kyung-Min Choi Seung-Hong Lee 

机构地区：[1]Department of Medical Science,Soonchunhyang University,Asan 31538,Republic of Korea [2]Honam National Institute of Biological Resources,99,Gohadoan-gil,Mokpo-si,Jeollanam-do 58762,Republic of Korea [3]Department of Pharmaceutical Engineering,Soonchunhyang University,Asan 31538,Republic of Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第4期137-146,共10页亚太热带生物医学杂志（英文版）

基　　金：This work was supported financially by Korea Environment Industry&Technology Institute through Project to make multi-ministerial national biological research resources more advanced program,funded by Korea Ministry of Environment(grant number RS-2023-00230403).

摘　　要：Objective:To evaluate the effect of the ethyl acetate fraction derived from Sargassum pallidum extract against particulate matter(PM)-induced oxidative stress and inflammation in HaCaT cells and zebrafish.Methods:HaCaT cells and zebrafish were used to evaluate the protective effects of the ethyl acetate fraction of Sargassum pallidum extract against PM-induced oxidative stress and inflammation.The production of nitric oxide(NO),intracellular ROS,prostaglandin E_(2)(PGE_(2)),and pro-inflammatory cytokines,and the expression levels of COX-2,iNOS,and NF-κB were evaluated in PM-induced HaCaT cells.Furthermore,the levels of ROS,NO,and lipid peroxidation were assessed in the PM-exposed zebrafish model.Results:The ethyl acetate fraction of Sargassum pallidum extract significantly decreased the production of NO,intracellular ROS,and PGE_(2) in PM-induced HaCaT cells.In addition,the fraction markedly suppressed the levels of pro-inflammatory cytokines and inhibited the expression levels of COX-2,iNOS,and NF-κB.Furthermore,it displayed remarkable protective effects against PM-induced inflammatory response and oxidative stress,represented by the reduction of NO,ROS,and lipid peroxidation in zebrafish.Conclusions:The ethyl acetate fraction of Sargassum pallidum extract exhibits a protective effect against PM-induced oxidative stress and inflammation both in vitro and in vivo and has the potential as a candidate for the development of pharmaceutical and cosmeceutical products.

关 键 词：Particulate matter INFLAMMATION Oxidative stress Sargassum pallidum Ethyl acetate fraction ZEBRAFISH 

分 类 号：R285.5[医药卫生—中药学]