爱必妥对前列腺癌PC-3细胞功能学的影响  

Study on the Functional Effects of Cetuximab on Prostate Cancer PC-3 Cells

作　　者：于卓玄 姜树旭 李昶毅 何东岳 赫振 傅德望 Yu Zhuoxuan;Jiang Shuxu;Li Changyi;He Dongyue;He Zhen;Fu Dewang(Department of Urinary Surgery,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000 China)

机构地区：[1]锦州医科大学附属第一医院泌尿外科,辽宁锦州121000

出　　处：《锦州医科大学学报》2024年第2期24-29,共6页Journal of Jinzhou Medical University

基　　金：锦州医科大学大学生创新创业训练计划项目,项目编号:x202210160076。

摘　　要：目的观察不同浓度爱必妥对前列腺癌PC-3细胞的增殖、侵袭及凋亡的作用,并初步探索其可能的作用机制。方法使用不同浓度的(10、20、40、80μg/mL)爱必妥作用于PC-3细胞24 h后,CCK-8检测细胞增殖活力变化;流式检测细胞凋亡变化;transwell检测细胞侵袭能力变化;RT-PCR检测EGFR mRNA表达变化;Western Blot法检测Bax和Bcl-2蛋白表达变化。结果CCK-8结果显示,不同浓度的爱必妥作用24 h后均可以抑制PC-3的细胞增殖活力(P<0.001),且药物抑制作用与浓度呈剂量依赖关系;凋亡检测结果显示,不同浓度的爱必妥均抑制PC-3细胞的侵袭能力(P<0.001),且药物抑制作用与浓度呈剂量依赖关系;凋亡检测结果显示,不同浓度的爱必妥均导致PC-3细胞的总凋亡比例增加(P<0.01),且这种促凋亡能力与与浓度呈剂量依赖关系;Western Blot结果显示,不同浓度的爱必妥均可以抑制Bc1-2蛋白表达(P<0.05),同时促进Bax蛋白表达(P<0.05),且抑制和促进作用与药物浓度呈剂量依赖关系;RT-PCR结果显示,不同浓度的爱必妥均可以抑制EGFR mRNA表达(P<0.05),且抑制作用与药物浓度同样呈剂量依赖关系。结论爱必妥可以抑制前列腺癌细胞PC-3的增殖和侵袭,促进细胞凋亡,且这种作用可能是通过抑制EGFR信号通路导致细胞凋亡而实现。Objective To observe the effects of different concentrations of cetuximab on the proliferation,invasion and apoptosis of prostate cancer PC-3 cells,and to explore its possible mechanism.Methods PC-3 cells were treated with different concentrations of cetuximab(10,20,40,and 80μg/mL)for 24 h,and CCK-8 was used to detect changes in cell proliferation activity;flow cytometry was used to detect changes in cell apoptosis;transwell assay was used to detect the changes in cell invasion ability;RT-PCR was used to detect changes in EGFR mRNA expression;Western Blot was used to detect changes in Bax and Bcl-2 protein expression.Results The CCK-8 results showed that different concentrations of cetuximab could inhibit the cell proliferation activity of PC-3 after 24 hours of action(P<0.001),and the drug′s inhibitory effect was dose-dependent on the concentration;the results of apoptosis detection showed that different concentrations of cetuximab inhibited the invasive ability of PC-3 cells(P<0.001),and the drug′s inhibitory effect was dose-dependent on the concentration;the apoptosis detection results showed that different concentrations of cetuximab resulted in an increase in the total apoptosis proportion of PC-3 cells(P<0.01),and this proapoptotic ability was dose-dependent on the concentration;Western Blot results showed that different concentrations of cetuximab could inhibit Bc1-2 protein expression(P<0.05)and promote Bax protein expression(P<0.05),and the inhibitory and promoting effects were dose-dependent on drug concentration;the RT-PCR results showed that different concentrations of cetuximab could inhibit EGFR mRNA expression(P<0.05),and the inhibitory effect was also dose-dependent on the drug concentration.Conclusion Cetuximab can inhibit the proliferation and invasion of PC-3 in prostate cancer cells and promote cell apoptosis,and this effect may be achieved by inhibiting EGFR signaling pathway leading to cell apoptosis.

关 键 词：前列腺癌 PC-3 爱必妥 EGFR 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]