在线活细胞定量检测模型的建立及其在辅酶Q10中的应用  

Establishment of online living cell quantitative detection model and its application in coenzyme Q10

作　　者：葛燕 刘建镔 翟辰欣 杨冰莹 郝喆江 王泽建 梁剑光[1] GE Yan;LIU Jianbin;ZHAI Chenxin;YANG Bingying;HAO Zhejiang;WANG Zejian;LIANG Jianguang(School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou 213164;Huzhou Laohenghe Brewing Co.,Ltd.,Huzhou 313000;State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200234)

机构地区：[1]常州大学药学院生物与食品工程学院,常州213164 [2]湖州老恒和酿造有限公司,湖州313000 [3]华东理工大学生物反应器工程国家重点实验室,上海200234

出　　处：《中国食品添加剂》2024年第4期219-226,共8页China Food Additives

基　　金：江苏省研究生科研与实践创新计划项目(项目编号:SJCX23_1456)。

摘　　要：在类球红细菌发酵生产辅酶Q10的过程中,其菌体生长代谢情况及产物合成与有活力的细胞量密切相关。活细胞传感仪通过利用细胞的介电特性,能够检测发酵液中的活细胞量,且检测结果不受死细胞、气泡的影响。在辅酶Q10的发酵过程中,运用活细胞传感仪采用全频扫描模式对检测到的电容值进行校正,校正后的电容值与CFU具有良好的线性关系,R^(2)=0.995,可以准确预测发酵液中菌体生长状态和活细胞量。基于电导率和校正后的电容值对辅酶Q10发酵过程中补料策略进行优化,辅酶Q10产量最终达到3420 mg/L。During the production of coenzyme Q10 by Rhodobacter sphaeroides,its growth and metabolism and the synthesis of product are closely related to the number of active cells.Using the dielectric properties of cells,the living cell sensor can detect the amount of living cells in the fermentation broth,and the detection results are not affected by dead cells and bubbles.In the fermentation process of coenzyme Q10,the on-line biomass monitor was used to correct the detected capacitance value in the full-frequency scanning mode.The corrected capacitance value had a good linear relationship with CFU,R^(2)=0.995,which could accurately predict the growth state of bacteria and the number of living cells in the fermentation broth.Based on the conductivity and the corrected capacitance value,the feeding strategy in the fermentation process of coenzyme Q10 was optimized,and the yield of coenzyme Q10 finally reached 3420 mg/L.

关 键 词：辅酶Q10 活细胞量 在线活细胞传感仪 电容值 电导率 

分 类 号：TS207.4[轻工技术与工程—食品科学] Q815[轻工技术与工程—食品科学与工程]