香料烟多糖提取工艺响应面优化及其抗氧化活性研究  

Study on Optimization of Extraction Technology and Antioxidant Activity of Polysaccharide from Aromatic Tobacco

作　　者：赵基仓 杨兆荣 李阿倩 何锐 李丽芬 赵红梅 ZHAO Jicang;YANG Zhaorong;LI Aqian;HE Rui;LI Lifen;ZHAO Hongmei(College of Science,Yunnan Agricultural University,Kunming Yunnan 650201,China;Fruit Tree Station,Luxi County,Honghe Prefecture Yunnan 661400,China)

机构地区：[1]云南农业大学理学院,云南昆明650201 [2]红河州泸西县果树站,云南红河州652499

出　　处：《江西化工》2024年第2期76-81,共6页Jiangxi Chemical Industry

基　　金：云南省教育厅科学研究基金项目(2023J0416)。

摘　　要：目标:优化云南保山香料烟多糖的提取工艺,并研究其体外抗氧化活性,为后期纯化香料烟多糖和卷烟加工奠定基础。方法:以多糖提取率为评价指标,在单因素实验的基础上采用响应面法对超声功率、超声时间、提取温度三个主要因素进行优化,得出最佳提取工艺。通过DPPH自由基、ABTS自由基清除和邻二氮菲法对体外清除自由基能力进行分析。结果:优化后多糖的最佳超声提取条件为时间80 min,温度为70℃,功率70 W,多糖提取率为3.02%。香料烟多糖浓度在0~5 mg/m L时,对羟自由基清除率不断提升,最高达71.26%,且IC50值为2.319 mg/m L;香料烟多糖浓度在0~10 mg/m L范围内增加时,DPPH的去除率先增加然后降低,IC50值为4.247 mg/m L,ABTS清除率随着多糖溶液浓度增加而升高。IC50值为6.418 mg/m L。Objectives:The purpose of this study was to optimize the extraction process of tobacco polysaccharides and to further explore their antioxidant activities.Methods:1.The effects of ultrasonic power,ultrasonic time and extraction temperature on the extraction rate of tobacco polysaccharides were studied.And the optimal extraction conditions were obtained by response surface optimization.DPPH radical scavenging,ABTS radical scavenging and o-diazepine scavenging were used to analyze the scavenging ability of free radicals in vitro.Results:The optimal ultrasonic extraction conditions were determined as follows:time 80min,temperature 70℃,power 70W.The extraction rate was 3.02%.When the concentration of aromatic tobacco polysaccharide was the range of 0~5 mg/mL,the scavenging rate of hydroxyl free radical increased continuously,reaching 71.26%,and the IC50 value was 2.319 mg/mL.The removal rate of DPPH increased and then decreased with IC50 of 4.247 mg/mL when the concentration of tobacco polysaccharide increased in the range of 0~10 mg/mL,the ABTS clearance rate increased with the increase of polysaccharide solution concentration.The IC50 value was 6.418 mg/mL.

关 键 词：香料烟 多糖 响应面 提取 抗氧化活性 

分 类 号：TS202[轻工技术与工程—食品科学] O629.1[轻工技术与工程—食品科学与工程]