高产苯乳酸菌株的筛选及发酵条件的优化  

Screening of high-yielding benzoilactic acid strains and optimization of fermentation conditions

作　　者：高阿波 李留安[1] 胡鹏程 刘佳琦 魏怡 刘鼎阔 GAO Abo;LI Liuan;HU Pengcheng;LIU Jiaqi;WEI Yi;LIU Dingkuo(Tianjin Agricultural College,Tianjin 300384,China;Dingzheng Xinxing Biotechnology(Tianjin)Co,Tianjin 300383,China;Tianjin Key Laboratory of Biological Feed Additives,Tianjin 300383,Chin)

机构地区：[1]天津农学院,天津300384 [2]鼎正新兴生物技术(天津)有限公司,天津300383 [3]天津市生物饲料添加剂重点实验室,天津300383

出　　处：《中国饲料》2024年第9期45-52,共8页China Feed

摘　　要：苯乳酸既可以延长饲料保质期又可以提高畜禽生产性能,但目前生物工业化生成苯乳酸的产量很低。为找到高产的菌株,本试验从泡菜中筛选出一株高产苯乳酸菌株,经16S rDNA测序确定该株为乳酸菌属植物乳杆菌。通过单因素试验与正交试验优化其发酵条件,由响应面试验确定外源添加物与温度之间的最佳方案,得到苯乳酸高产方案。结果表明:正交试验和响应面试验确定葡萄糖添加量为25 g/L、玉米浆添加量为5 g/L、接种量为100μL菌液、发酵时间为72 h、苯丙氨酸添加量为8 g/L、柠檬酸添加量为5 g/L、反应温度为33℃时苯乳酸的表达量达到374.26 mg/L,优于未优化前38.97 mg/L的产量。在优化后的植物乳杆菌可以高产苯乳酸,解决原始株表达量低的问题。Phenyl lactic acid can both extend the shelf life of feed and improve the performance of livestock and poultry.However,the current yield of bioindustrial production of phenyl lactic acid is very low.To find a high-yielding strain,a high-yielding phenyl lactic acid strain was screened from kimchi and identified as Lactobacillus plantarum by 16S rDNA sequencing.The fermentation conditions were optimized by single-factor test and orthogonal test,and the optimal solution between exogenous additive and temperature was determined by response surface test to obtain the high yield of phenyl lactic acid.The results showed that the orthogonal and response surface tests determined that the expression of phenyllactic acid reached 374.26 mg/L with 25 g/L of glucose addition,5 g/L of corn pulp addition,100μL of inoculum,72 h of fermentation time,8 g/L of phenylalanine addition,5 g/L of citric acid addition,and 33℃of reaction temperature,which was better than that of 38.97 mg/L before optimization.The optimized Lactobacillus plantarum could produce high benzolactic acid and solve the problem of low expression of the original strain.

关 键 词：苯乳酸 植物乳杆菌 苯丙氨酸 正交试验 响应面 

分 类 号：S816.3[农业科学—饲料科学]