裂解多糖单加氧酶的研究进展  

Research progress on lytic polysaccharide monooxygenases

作　　者：陈凌宇 李志建[1] 刁文涛[2,3] 王佰涛 刘德海[2] CHEN Lingyu;LI Zhijian;DIAO Wentao;WANG Baitao;LIU Dehai(Collage of Food and Engineering,Henan University of Technology,Zhengzhou 450001,China;Institute of Biology Co.Ltd.,Henan Academy of Science,Zhengzhou 450008,China;Key Laboratory of Microbial Engineering of Henan,Zhengzhou 450008,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]河南省科学院生物研究所有限责任公司,河南郑州450008 [3]河南省微生物工程重点实验室,河南郑州450008

出　　处：《食品与发酵工业》2024年第8期393-400,共8页Food and Fermentation Industries

基　　金：河南省科技研发计划联合基金项目(225200810041);河南省科学院功能微生物绿色转化创新团队(20230114)。

摘　　要：多糖类化合物在自然界中广泛存在,但目前针对多糖类底物的酶解效果并不理想。裂解多糖单加氧酶(lytic polysaccharide monooxygenase,LPMO)是一种新型的多糖降解酶,其能够以氧化方式打开纤维素中的糖苷键使其出现更多的酶解位点,从而在降解纤维素等多糖类物质中发挥着巨大优势。该文从LPMO的分类、结构、活性测定方法、异源表达及其在食品、饲料等领域的应用等方面进行了介绍,以期为该酶的推广应用提供帮助。Polysaccharide compounds are widely present in nature,but the enzymatic hydrolysis effect on polysaccharide substrates is currently not ideal.The lytic polysaccharide monooxygenase(LPMO)is a novel polysaccharide-degrading enzyme that can open glycosidic bonds in cellulose through oxidation,resulting in more enzymatic hydrolysis sites and playing a great advantage in degrading polysaccharides such as cellulose.The paper introduces the classification,structure,activity determination methods,heterologous expression,and application of LPMO in food,feed,and other fields,aiming to provide assistance for the promotion and application of this enzyme.

关 键 词：裂解多糖单加氧酶 生物能源 纤维素酶促进剂 多糖降解 

分 类 号：TQ925[轻工技术与工程—发酵工程]