2021-2023年广东省食物中毒来源金黄色葡萄球菌耐药特征与全基因组分析  

Antibiotic resistance characteristics and genome-wide analysis of Staphylococcus aureus in food poisoning cases in Guangdong Province,2021-2023

作　　者：李亭 何冬梅[1,3] 柯碧霞 欧阳方竹[1,3] 徐静 李柏生 颜瑾[1,3] LI Ting;HE Dongmei;KE Bixia;OUYANG Fangzhu;XU Jing;LI Bosheng;YAN Jing(Institute of Pathogenic Microbiology,Guangdong Centre for Disease Control and Prevention,Guangzhou 510310,China;Department of Epidemiology and Health Statistics,School of Public Health,Guangdong Pharmaceutical University;Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response)

机构地区：[1]广东省疾病预防控制中心病原微生物所,广东广州510310 [2]广东药科大学公共卫生学院流行病与卫生统计学系 [3]广东省新发传染病应急检测技术研究与应用重点实验室

出　　处：《中国病原生物学杂志》2024年第5期534-538,共5页Journal of Pathogen Biology

摘　　要：目的分析2021-2023年广东省疾病预防控制中心收到的食物中毒来源金黄色葡萄球菌(Staphylococcus aureus,SA)耐药特点、分子特征与同源性。方法使用VITEK 2 Compact全自动微生物生化鉴定仪进行菌株鉴定。使用微量肉汤稀释法进行药敏试验。利用全基因组测序技术揭示菌株的分子流行特征。使用多位点序列分型(Multilocus sequence typing,MLST)与系统发育分析菌株间同源性。结果8起SA食物中毒事件主要发生在春季(62.5%,5/8);导致中毒的食物类型主要为肉蛋制品(87.5%,7/8)。耐药分析结果表明75株SA均对青霉素耐药,对其他10种药物大部分敏感(76.0%~100.0%);使用耐药基因ErmB、BlaZ、MecA、TetM、Ant6预测SA对红霉素、青霉素、苯唑西林、四环素与庆大霉素耐药性,其总体符合率较高(94.7%)。MLST分析结果显示8起食物中毒来源SA的主要ST型为ST7、ST6与ST45;同一起食物中毒事件来源株同源率为80.5%~100.0%,系统发育进一步提示不同地级市食物中毒来源SA存在传播现象。结论SA所致食物中毒事件春季高发,肉蛋制品为主要致病食物;食物中毒来源SA均对青霉素耐药,且携带多种毒素基因;同一起食物中毒来源SA同源性较高,不同地区食物中毒来源菌株存在传播现象。Objective This study aims to examine the antibiotic resistance profiles,molecular characteristics,and homology of Staphylococcus aureus(SA)strains associated with food poisoning cases reported to the Guangdong Provincial Center for Disease Control and Prevention from 2021 to 2023.Methods The VITEK 2 Compact fully automated microbiological and biochemical identifier was used for strain identification.Antimicrobial susceptibility was assessed using the broth microdilution method.Whole-genome sequencing provided the molecular epidemiological features of the strains.Multilocus sequence typing(MLST)and phylogenetic analyses were utilized to evaluate strain homology.Results The majority of the eight SA food poisoning incidents occurred during the spring season(62.5%,5/8),with the consumption of meat and egg products as the predominant reason(87.5%,7/8).Antibiotic resistance testing showed that all 75 SA strains were resistant to penicillin,but largely sensitive to the other ten antibiotics tested(Antibiotic susceptibility rate ranged from 76.0%to 100.0%).Predictive concordance for resistance to erythromycin,penicillin,oxacillin,tetracycline,and streptomycin using resistance genes ErmB,BlaZ,MecA,TetM,and Ant6 was notably high,with the predicted compliance rates being 97.3%,90.7%,96.0%,89.3%,and 100.0%,respectively(the combined rate was 94.7%).MLST revealed the primary STs of the food poisoning strains to be ST7(41.3%),ST6(21.3%),and ST45(14.7%).Homology rates among strains from the different food poisoning cases ranged from 80.5%to 100.0%.Phylogenetic analysis indicated the presence of transmission phenomena among SA strains from different prefecture-level cities.Conclusion Incidents of SA-induced food poisoning were found to peak in the spring,with consumption of meat and egg products being the principal modes of transmission.All SA strains from the food poisoning cases demonstrated penicillin resistance and harbored multiple toxin genes.Strains from the same incident exhibited a high degree of homology,while transmiss

关 键 词：金黄色葡萄球菌 食物中毒 药物耐受性 全基因组测序 毒力因子 系统发育分析 

分 类 号：R155.3[医药卫生—营养与食品卫生学]