Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance  

作　　者：Sujin Lee Yeunjung Ko Hyun Woo Lee Won Joon Oh Hun Gi Hong Dinuka Ariyaratne Se Jin Im Tae Jin Kim 

机构地区：[1]Department of Immunology,Graduate School of Basic Medical Science,Sungkyunkwan University School of Medicine,Suwon,16419,Republic of Korea [2]Department of Immunology and Molecular Medicine,Faculty of Medical Sciences,University of Sri Jayewardenepura,Nugegoda,Sri Lanka [3]Present address:Immunology and Microbiology Graduate Program,Baylor College of Medicine,Houston,TX,77030,USA

出　　处：《Cellular & Molecular Immunology》2024年第4期393-408,共16页中国免疫学杂志（英文版）

基　　金：supported by National Research Foundation of Korea grant funded by the Korea government(MSIT)(2023R1A2C2004510);Korea Basic Science Institute(National Research Facilities and Equipment Center)grant(2020R1A6C101A191)of the Ministry of Education(Korea);the BK21 FOUR Program(Graduate School Innovation)of Sungkyunkwan University.

摘　　要：Marginal zone(MZ)B cells,which are splenic innate-like B cells that rapidly secrete antibodies(Abs)against blood-borne pathogens,are composed of heterogeneous subpopulations.Here,we showed that MZ B cells can be divided into two distinct subpopulations according to their CD80 expression levels.CD80^(high)MZ B cells exhibited greater Ab-producing,proliferative,and IL-10-secreting capacities than did CD80^(low)MZ B cells.Notably,CD80^(high)MZ B cells survived 2-Gy whole-body irradiation,whereas CD80^(low)MZ B cells were depleted by irradiation and then repleted with one month after irradiation.Depletion of CD80^(low)MZ B cells led to accelerated development of type II collagen(CII)-induced arthritis upon immunization with bovine CII.CD80^(high)MZ B cells exhibited higher expression of genes involved in proliferation,plasma cell differentiation,and the antioxidant response.CD80^(high)MZ B cells expressed more autoreactive B cell receptors(BCRs)that recognized double-stranded DNA or CII,expressed more immunoglobulin heavy chain sequences with shorter complementarity-determining region 3 sequences,and included more clonotypes with no N-nucleotides or with B-1a BCR sequences than CD80^(low)MZ B cells.Adoptive transfer experiments showed that CD21^(+)CD23^(+)transitional 2 MZ precursors preferentially generated CD80^(low)MZ B cells and that a proportion of CD80^(low)MZ B cells were converted into CD80^(high)MZ B cells;in contrast,CD80^(high)MZ B cells stably remained CD80^(high)MZ B cells.In summary,MZ B cells can be divided into two subpopulations according to their CD80 expression levels,Ab-producing capacity,radioresistance,and autoreactivity,and these findings may suggest a hierarchical composition of MZ B cells with differential stability and BCR specificity.

关 键 词：Marginal Zone B Cell CD80 AUTOREACTIVITY RADIORESISTANCE 

分 类 号：R392[医药卫生—免疫学]