体外模拟胃肠消化过程中云南金花茶抗氧化能力变化  

Changes in the Antioxidant Capacity of Yunnan Camellia fascicularis during in Vitro Simulated Gastrointestinal Digestion

作　　者：石志娇 唐军荣[1] 向建英[1] 阚欢[1,2] 赵平 张贵良[3] 刘云 SHI Zhijiao;TANG Junrong;XIANG Jianying;KAN Huan;ZHAO Ping;ZHANG Guiliang;LIU Yun(Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education,Southwest Forestry University,Kunming 650224,China;Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities,Kunming 650224,China;Hekou Management Sub-bureau of Yunnan Daweishan National Nature Reserve Management Bureau,Hekou 661399,China)

机构地区：[1]西南林业大学西南山地森林资源保育与利用教育部重点实验室,云南昆明650224 [2]云南省高校大健康类森林资源开发利用工程研究中心,云南昆明650224 [3]云南大围山国家级自然保护区管护局河口管护分局,云南河口661399

出　　处：《现代食品科技》2024年第4期95-103,共9页Modern Food Science and Technology

基　　金：云南省基础研究计划项目(202201AT070050);云南省教育厅科学研究基金项目(2023Y0789);云南省“兴滇英才支持计划”青年人才专项(XDYC-QNRC-2022-0222);云南省农业基础研究联合专项(202101BD070001-045);国际植物园联盟项目(2063076)。

摘　　要：为探究云南金花茶在消化过程中总黄酮、总多酚释放及功能活性的变化情况,该研究采用体外模拟胃肠消化模型测定了云南金花茶消化液中总黄酮、总多酚浓度及自由基清除能力、铁离子还原能力和脂质过氧化抑制能力,并对其进行了相关性分析。结果表明,模拟胃消化过程中,云南金花茶总黄酮、总多酚含量均比口腔消化高,最高分别达229.99、327.65µg/mL,经肠消化后,总黄酮含量在38.56~78.88µg/mL之间,总多酚含量在30.04~65.52µg/mL之间,含量较低。在功能活性测定中,较口腔消化,胃消化阶段的DPPH·、ABTS+·清除能力和铁离子还原能力显著升高,脂质过氧化抑制能力随消化的进行呈上下波动;经肠消化后,DPPH·、ABTS+·清除能力和铁离子还原能力急剧下降,且均比胃消化阶段低,而脂质过氧化抑制能力则趋于平稳或逐渐上升。通过相关性及主成分分析,各指标之间存在一定的关联性。综上所述,云南金花茶是较好的活性物质来源,该研究可为探索云南金花茶在人体消化过程中活性物质的变化及作用机制提供一定的实验基础和新思路。To investigate the changes in the release and functional activity of total flavonoids and total polyphenols of Camellia fascicularis during the digestion,the total flavonoid and total polyphenol contents,as well as free radical scavenging ability,iron ion reducing ability,and lipid peroxidation inhibitory ability of the C.fascicularis digestive fluid were determined using an in vitro simulated gastrointestinal digestion model,and the analysis of their correlation was performed.The results showed that during simulated gastric digestion,the total flavonoid and total polyphenol contents of C.fascicularis were higher than thoseduring oral digestion,with the highest values reaching 229.99 and 327.65µg/mL,respectively.After the intestinal digestion,the total flavonoid and total polyphenol contents were both low,ranging from 38.56 to 78.88µg/mL,and 30.04 to 65.52µg/mL,respectively.In the functional activity assay,DPPH and ABTS+·scavenging abilities and iron ion reducing ability were significantly higher in the gastric digestion phase than in the oral digestion,while the lipid peroxidation inhibitory ability fluctuated with the progress of digestion.After the intestinal digestion,DPPH and ABTS+·scavenging abilities and iron ion reducing ability decreased sharply after the intestinal digestion,which were lower than those during gastric digestion,whilst the lipid peroxidation inhibitory ability tended to be stable or increased gradually.The correlation analysis and principal component analysis revealed that there was certain correlations between the indicators.In summary,C.fascicularis is a better source of active substances.This study can provide a certain experimental basis and new ideas for exploring the changes of active substances and their working mechanisms during human digestion of C.fascicularis.

关 键 词：云南金花茶 模拟胃肠道消化 清除自由基 抑制脂质过氧化 

分 类 号：TS272.53[农业科学—茶叶生产加工]