食用酵母提取物通过上调Nrf-2改善皮肤衰老模型小鼠抗氧化水平  

Edible yeast extract improves antioxidant levels through up regulation of Nrf-2 in skin aging model mice

作　　者：付少委 秦修远 张卓然 李明亮 冯志远 栾金玲 任玮 程倩 FU Shaowei;QIN Xiuyuan;ZHANG Zhuoran;LI Mingliang;FENG Zhiyuan;LUAN Jinling;REN Wei;CHENG Qian(China National Research Institute of Foodand Fermentation Industries Co.Ltd.,Beijing 100015,China;Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015,China;Hubei Key Laboratory of Yeast Function,Yichang Engineering Technology Research Center of Nutrition and Health Food,Yichang 443003,China)

机构地区：[1]中国食品发酵工业研究院有限公司,北京100015 [2]北京市蛋白功能肽技术研究中心,北京100015 [3]酵母功能湖北省重点实验室宜昌市营养健康食品工程技术研究中心,湖北宜昌443003

出　　处：《食品与发酵工业》2024年第9期17-22,共6页Food and Fermentation Industries

基　　金：安琪纽特营养基金项目(AF2021004);中轻集团科技创新基金项目(ZQ2022JC-QN09);宁夏回族自治区重点研发计划项目(2021BEG02027)。

摘　　要：酵母提取物是一种天然安全的活性物质,作为抗氧化剂在食品工业中具有广泛应用。探究食用酵母提取物对皮肤抗衰老的功效作用。该研究采用D-半乳糖诱导构建皮肤衰老动物模型,利用不同剂量的酵母提取物进行干预,雄性ICR小鼠随机分为对照组(Control)、模型组(Model)、阳性组(GSH)、酵母提取物低剂量组(LYE)、酵母提取物中剂量组(MYE)、酵母提取物高剂量组(HYE),每组12只,连续处理70 d,每周进行称重。末次灌胃后禁食12 h,称重,随后对小鼠进行眼眶取血用于氧化应激指标检测,收集小鼠皮肤组织用于形态学观察,免疫蛋白印迹法检测皮肤组织Nrf-2蛋白的表达量。酵母提取物实验组小鼠皮肤组织表皮层增厚情况有明显改善,血清中的谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性和丙二醛(malondialdehyde,MDA)含量随着酵母提取物剂量的增加,抗氧化水平也进一步提高,逐渐趋于空白组水平,并通过上调Nrf-2蛋白来减轻衰老导致的氧化应激反应。该研究得出食用酵母提取物主要是从减轻抗氧化应激途径来实现抗衰老作用,同时也得出了具有改善效果的最佳服用量。Yeast extract is a natural and safe active substance that is widely used as an antioxidant in the food industry.To investigate the efficacy effect of edible yeast extract on anti-aging of skin.In this study,D-galactose was used to induce the construction of an animal model of skin aging,and different doses of yeast extract were used to intervene.Male ICR mice were randomly divided into control group(Control),model group(Model),positive group(GSH),low-dose group of yeast extract(LYE),medium-dose group of yeast extract(MYE),and high-dose group of yeast extract(HYE).Twelve animals in each group were treated for 70 consecutive days and weighed weekly.After the final gavage,the mice were fasted for 12 hours and weighed,followed by orbital blood sampling for oxidative stress indexes,and skin tissues were collected for morphological observation of skin tissues,and the expression of Nrf-2 protein in skin tissues was detected by immunoprotein blotting.The thickening of the epidermal layer of the skin tissue of mice in the experimental group of yeast extract showed significant improvement,and the serum GSH-Px enzyme activity and MDA content further increased with the increase of yeast extract dose,and the antioxidant level gradually converged to the level of the blank group,and attenuated the oxidative stress response caused by aging through up-regulation of Nrf-2 protein.This study concluded that the consumption of yeast extracts furthered the anti-aging effect mainly from the alleviation of antioxidant stress pathway,and also derived the optimal dosage with improved effects.

关 键 词：酵母提取物 抗衰老 抗氧化 Nrf-2 

分 类 号：TS201.4[轻工技术与工程—食品科学]