虾青素工程菌中核酸的脱除工艺研究  

Study on the removal of nucleic acid from astaxanthin engineered bacteria

作　　者：郝孔利 张策 于京鹏 初文琴 梁寒冰 Hao Kongli;Zhang Ce;Yu Jingpeng;Chu Wenqin;Liang Hanbing(Shandong Jincheng Biopharmaceutical Co.,Ltd,Shandong Zibo 255100)

机构地区：[1]山东金城生物药业有限公司,山东淄博255100

出　　处：《现代畜牧兽医》2024年第2期15-18,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

摘　　要：试验旨在探究研究红法夫酵母菌中核酸的脱除工艺,选取了核酸清除剂、核酸酶和十六烷基三甲基溴化铵(cetyltrimethylammonium bromide,CTAB)和NaCl脱除红法夫酵母菌核酸,通过检测上清液核酸脱除率、虾青素含量以及上清液与菌体PCR结果,得出核酸脱除的最佳工艺。结果显示,核酸脱除最佳工艺为:发酵液菌浓为20%,菌悬液1 mL加233μL NaCl(6 mol/L)和161μL的CTAB/NaCl,65℃搅拌30 min,该工艺核酸脱除率80%。研究表明,CTAB/NaCl方法脱除红法夫酵母菌核酸的效果最佳。The aim of the experiment was to study the removal process of nucleic acid from Phaffia rhodozyma.Nucleic acid scavengers,nucleases,CTAB and NaCl were selected to remove nucleic acid from Phaffia rhodozyma.The optimal process of nucleic acid removal was obtained by detecting the rate of nucleic acid removal and astaxanthin concentrations in supernatant and PCR results between supernatant and bacteria.The results showed that the optimal process of nucleic acid removal was as follows:The fermentation solution was 20%concentrated,and the suspensions were mixed with 1 mL of 233μL NaCl(6 mol/L)and 161μL CTAB/NaCl at 65℃for 30 min.The removal rate of nucleic acid was 80%.The results showed that the CTAB/NaCl method had the best effect of removing nucleic acid from Phaffia rhodozyma.

关 键 词：虾青素 红法夫酵母 工程菌 核酸 

分 类 号：S816[农业科学—饲料科学]