咀嚼型洁齿骨活性成分体外抑菌试验和临床应用效果评估  

In Vitro Antibacterial Test and Clinical Application Effectiveness Evaluation of Active Ingredients for Chewable Tooth-Cleaning Bone

作　　者：卢玲[1] 尹一茹 李向宝 许灵 毛新 王媛媛[1] 魏学良[1] LU Ling;YIN Yiru;LI Xiangbao;XU Ling;MAO Xin;WANG Yuanyuan;WEI Xueliang(College of Veterinary Medicine,Southwest University,Chongqing 400715,China;Chongqing Siweite Pet Products Co.,Ltd.,Chongqing 402160,China)

机构地区：[1]西南大学动物医学院,重庆北碚400715 [2]重庆思味特宠物用品股份有限公司,重庆永川402160

出　　处：《中国兽医杂志》2024年第5期136-140,共5页Chinese Journal of Veterinary Medicine

基　　金：重庆市技术创新与应用发展专项(cstc2019jscx-msxmX0396)。

摘　　要：为了评估咀嚼型洁齿骨对成年犬口腔健康状况的改善情况及其主要活性成分的体外抑菌效果,本试验采用牛津杯法定性检测咀嚼型洁齿骨中主要活性成分对变异链球菌、粘性放线菌、嗜酸乳杆菌、牙龈卟啉单胞菌和具核梭杆菌具核亚种的抑菌活性;并选取30只临床健康成年犬,经超声波洁牙后,随机分为3个组,对照组正常饲喂,试验1组和试验2组分别添加不同品牌的洁齿骨,每间隔30 d由同一名兽医对试验犬的牙菌斑、牙结石和牙龈炎进行医学评分并测定犬口气值。结果显示:咀嚼型洁齿骨中活性成分茶多酚、厚朴酚、杜仲提取物、金银花提取物、溶菌酶和保鲜复合酶对5种口腔常见致病菌的生长抑制效果相对较好;洁牙后90 d,试验1组和试验2组犬牙菌斑和牙结石评分均显著低于对照组(P<0.05);洁牙后30和90 d,试验2组犬牙龈炎评分显著低于对照组(P<0.05);与对照组相比,洁牙后饲喂咀嚼型洁齿骨明显改善了犬口臭情况,试验2组犬口气测定值均低于试验1组,差异不显著(P>0.05)。结果表明,咀嚼型洁齿骨一定程度上可以缓解犬口腔健康状况,且添加活性成分的洁齿骨效果相对较好。In order to assess the improvement of oral health in adult dogs and the in vitro antibacterial effect of the main active ingredients of chewable tooth-cleaning bones,this study qualitatively detected the inhibitory activity of the main active ingredients in chewable tooth-cleaning bones against Streptococcus mutans,Actinomyces viscosus,Lactobacillus acidophilus,Porphyromonas gingivalis,and Fusobacterium nucleatum subsp.nucleatum using the Oxford cup method.Thirty clinically healthy adult dogs were selected and randomly divided into three groups.After ultrasonic teeth cleaning,the Control group was fed normally,while Test groups 1 and 2 were fed with chewable tooth-cleaning bones of different brands.Every 30 days,the same veterinarian evaluated the dental plaque,calculus,and gingivitis of the test dogs and measured their breath odor.The results showed that the active ingredients in chewable tooth-cleaning bones,such as tea polyphenol,magnolol,Eucommia ulmoides extract,honeysuckle extract,lysozyme,and preservative complex enzyme,had relatively good inhibitory effects on the growth of five common oral pathogens.After 90 days of teeth cleaning,the dental plaque and tartar scores of Test groups 1 and 2 were significantly lower than those of the Control group(P<0.05).After 30 and 90 days of teeth cleaning,the gingivitis scores of Test group 2 were significantly lower than those of the Control group(P<0.05).Compared with the Control group,feeding chewable tooth-cleaning bones after teeth cleaning significantly improved the breath odor of the dogs.The breath odor values of Test group 2 were lower than those of Test group 1,but the difference was not significant(P>0.05).These results indicate that chewable tooth-cleaning bones can alleviate the oral health of dogs to some extent,and tooth-cleaning bones with added active ingredients have relatively good effects.

关 键 词：咀嚼型洁齿骨 抑菌活性 口腔健康 口臭 

分 类 号：S854[农业科学—临床兽医学]