青果不同溶剂提取物对α-葡萄糖苷酶抑制作用研究  

Inhibition ofα-Glucosidase by Different Solvent Extracts of Qingguo(Canarii Fructus)

作　　者：张震宇 刘雅欣 刘国香 邵文卓 张德成 林桂涛[1] ZHANG Zhenyu;LIU Yaxin;LIU Guoxiang;SHAO Wenzhuo;ZHANG Decheng;LIN Guitao(School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Beijing School,Beijing 101100,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]北京学校,北京101100

出　　处：《山东中医药大学学报》2024年第3期348-354,共7页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省重点研发计划项目(编号:2016CYJS08A01-10)。

摘　　要：目的:探究青果不同溶剂提取物的成分差异及对α-葡萄糖苷酶的抑制效果。方法:使用水、50%乙醇、70%乙醇、95%乙醇对青果进行提取,分别测定不同提取物对α-葡萄糖苷酶的抑制作用,明确其抑制类型,并检测不同提取液中没食子酸、鞣花酸、总黄酮含量。结果:各溶剂提取物均对α-葡萄糖苷酶有不同程度的抑制作用,其抑制类型为可逆性非竞争性抑制作用;70%乙醇提取物抑制效果最佳,半抑制浓度(IC50)为9.914×10^(3)µg/mL,没食子酸、鞣花酸、总黄酮含量分别为(5.33±0.03)mg/g、(12.61±0.12)mg/g、(19.05±0.02)mg/g。结论:青果提取液成分中没食子酸对提取溶剂不敏感,鞣花酸及总黄酮水溶性不佳;对α-葡萄糖苷酶的抑制效果总体以70%乙醇提取液最佳,酶抑制率最高,此为青果开发提供了依据。Objective:To explore the composition differences of different solvents extracts of Qingguo(Canarii Fructus)and their inhibitory effects onα-glucosidase.Methods:Qingguo was extracted with water,50%ethanol,70%ethanol and 95%ethanol.The inhibitory effects of different extracts onα-glucosidase were determined to clarify their inhibitory types.The contents of gallic acid,ellagic acid and total flavonoids in different extracts were detected.Results:The solvent extracts had different degrees of inhibitory effects onα-glucosidase,and their inhibitory types were reversible noncompetitive inhibitory effects.The 70%ethanol extract had the best inhibitory effect,with IC50 of 9.914×10^(3)µg/mL.The contents of gallic acid,ellagic acid and total flavonoids were(5.33±0.03)mg/g,(12.61±0.12)mg/g and(19.05±0.02)mg/g,respectively.Conclusions:The gallic acid in Qingguo extracts is not sensitive to extraction solvents,and the ellagic acid and total flavonoids are poorly water soluble.The 70%ethanol extract had the best inhibition effect onα-glucosi⁃dase,and the enzyme inhibition rate was the highest,which provided a basis for the development of Qingguo.

关 键 词：青果 Α-葡萄糖苷酶 酶抑制 没食子酸 鞣花酸 总黄酮 

分 类 号：R289.1[医药卫生—方剂学]