Transcranial Magneto-Acoustic Stimulation Attenuates Synaptic Plasticity Impairment through the Activation of Piezo1 in Alzheimer’s Disease Mouse Model  

作　　者：Fangxuan Chu Ruxin Tan Xin Wang Xiaoqing Zhou Ren Ma Xiaoxu Ma Ying Li Ruixu Liu Chunlan Zhang Xu Liu Tao Yin Zhipeng Liu 

机构地区：[1]Institute of Biomedical Engineering,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin,300192,China [2]Tianjin Institutes of Health Science,Tianjin,301600,China [3]Neuroscience Center,Chinese Academy of Medical Science&Peking Union Medical College,Beijing,100730,China

出　　处：《Research》2024年第1期81-95,共15页研究（英文）

基　　金：the National Natural Science Foundation of China(81927806,52107241,and 52077223);Chinese Academy of Medical Sciences Medical and Health Science and Technology Innovation Project Mission Statement(2021-I2M-1-058);CAMS Innovation Fund for Medical Sciences(2022-I2M-2-003).

摘　　要：The neuropathological features of Alzheimer’s disease include amyloid plaques.Rapidly emerging evidence suggests that Piezo1,a mechanosensitive cation channel,plays a critical role in transforming ultrasound-related mechanical stimuli through its trimeric propeller-like structure,but the importance of Piezo1-mediated mechanotransduction in brain functions is less appreciated.However,apart from mechanical stimulation,Piezo1 channels are strongly modulated by voltage.We assume that Piezo1 may play a role in converting mechanical and electrical signals,which could induce the phagocytosis and degradation of Aβ,and the combined effect of mechanical and electrical stimulation is superior to single mechanical stimulation.Hence,we design a transcranial magneto-acoustic stimulation(TMAS)system,based on transcranial ultrasound stimulation(TUS)within a magnetic field that combines a magnetoacoustic coupling effect electric field and the mechanical force of ultrasound,and applied it to test the above hypothesis in 5xFAD mice.Behavioral tests,in vivo electrophysiological recordings,Golgi–Cox staining,enzyme-linked immunosorbent assay,immunofluorescence,immunohistochemistry,real-time quantitative PCR,Western blotting,RNA sequencing,and cerebral blood flow monitoring were used to assess whether TMAS can alleviate the symptoms of AD mouse model by activating Piezo1.

关 键 词：stimulation ALZHEIMER Piezo1 

分 类 号：R749.16[医药卫生—神经病学与精神病学]