生物信息学资源助力蛋白质磷酸化的系统层次理解  

Using bioinformatic resources for a systems-level understanding of phosphorylation

作　　者：彭迪 贾大 夏宏光 张珞颖 黄鹏宇 薛宇 Di Peng;Da Jia;Hongguang Xia;Luoying Zhang;Pengyu Huang;Yu Xue(Department of Bioinformatics and Systems Biology,MOE Key Laboratory of Molecular Biophysics,Hubei Bioinformatics and Molecular Imaging Key Laboratory,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China;Key Laboratory of Birth Defects and Related Diseases of Women and Children,Department of Paediatrics,West China Second University Hospital,State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China;Department of Biochemistry&Research Center of Clinical Pharmacy of The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China;Institute of Biomedical Engineering,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China;Nanjing University Institute of Artificial Intelligence Biomedicine,Nanjing 210031,China)

机构地区：[1]Department of Bioinformatics and Systems Biology,MOE Key Laboratory of Molecular Biophysics,Hubei Bioinformatics and Molecular Imaging Key Laboratory,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China [2]Key Laboratory of Birth Defects and Related Diseases of Women and Children,Department of Paediatrics,West China Second University Hospital,State Key Laboratory of Biotherapy,Sichuan University,Chengdu 610041,China [3]Department of Biochemistry&Research Center of Clinical Pharmacy of The First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310058,China [4]Institute of Biomedical Engineering,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin 300192,China [5]Nanjing University Institute of Artificial Intelligence Biomedicine,Nanjing 210031,China

出　　处：《Science Bulletin》2024年第8期989-992,共4页科学通报（英文版）

基　　金：supported by the National Key R&D Program of China(2022YFC2704300,2021YFF0702000 and 2021ZD0201300);the National Natural Science Foundation of China(32341020,32341021,and 31930021);Hubei Innovation Group Project(2021CFA005);Hubei Province Postdoctoral Outstanding Talent Tracking Support Program;the Research Core Facilities for Life Science(HUST)。

摘　　要：Protein phosphorylation,catalyzed by protein kinases(PKs),is one of the most prevalent post-translational modifications(PTMs)in eukaryotes.Phosphorylation occurring at different positions in a protein sequence can possess distinct functional impacts,and sitespecific phosphorylation may drastically alter a protein’s conformation,activity,and trafficking.Traditionally,biologists usually focused on studying regulatory mechanisms of phosphorylation in a limited number of proteins,mainly due to a lack of highthroughput technology.During the past two decades,advances in liquid chromatography coupled with tandem mass spectrometry(LC-MS/MC)have boomed a revolutionary technology named phosphoproteomics,which can simultaneously quantify thousands of phosphorylation sites(p-sites),and provide a great opportunity for a systems-level understanding of phosphorylation.Besides LCMS/MC,other approaches such as immunohistochemistry(IHC)and immune-detection by sequencing(ID-seq)have also emerged.

关 键 词：POSITIONS CATALYZED TRANSLATIONAL 

分 类 号：Q811.4[生物学—生物工程]