菠萝蜜多糖在DSS肠炎大鼠血液中的代谢标志物分析  

Metabolomics Analysis of JFP-Ps in Serum of DSS Colitis Rats

作　　者：陈玉子 李云龙 曾顺江 吴刚[1,4] 谭乐和 朱科学[1,4,5] Chen Yuzi;Li Yunlong;Zeng Shunjiang;Wu Gang;Tan Lehe;Zhu Kexue(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning 571533,Hainan;College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070;College of Food Science and Engineering,Hainan University,Haikou 570228;Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province,Wanning 571533,Hainan;Key Laboratory of Nutritional Quality and Health Benefits of Tropical Agricultural Products of Haikou City,Haikou 571100)

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]华中农业大学食品科学技术学院,武汉430070 [3]海南大学食品科学与工程学院,海口570228 [4]海南省特色热带作物适宜性加工与品质控制重点实验室,海南万宁571533 [5]海口市热带农产品营养品质与健康功效重点实验室,海口571100

出　　处：《中国食品学报》2024年第4期22-31,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：海南省重点研发计划项目(ZDYF2020218);中国热带农业科学院国家热带农业科学中心科技创新团队(CATASCXTD202304);中国热带农业科学院基本科研业务费专项资金(1630142022009)。

摘　　要：采用超高液相串联四极杆飞行时间质谱(UPLC-Q-TOF-MS/MS)分析菠萝蜜多糖(JFP-Ps)在DSS肠炎大鼠血清中代谢物全谱的变化,代谢组学分析技术探究JFP-Ps在DSS肠炎大鼠血液中的差异代谢标志物。结果表明,JFP-Ps主要引起氨基酸、多肽及其类似物、嘌呤和嘧啶及其衍生物、脂类和类脂分子等代谢物的变化。大鼠血清中与肠炎相关的代谢通路有5条,分别为精氨酸的生物合成、D-精氨酸和D-鸟氨酸代谢、苯丙氨酸代谢、苯丙氨酸、酪氨酸、色氨酸的生物合成、精氨酸和脯氨酸代谢途径。结论:JFP-Ps可通过调节氨基酸代谢及影响相关通路,改善DSS肠炎大鼠的症状。UPLC-Q-TOF-MS/MS was used to analyze the metabolite profiles of JFP-Ps(Polysaccharides from Artocarpus heterophyllus Lam.pulp)in the serum of DSS colitis rats.Then metabonomics analysis was used to explore the serum metabolite profiles and metabolic pathway.The results showed that JFP-Ps mainly regulated these metabolites such as amino acids,peptides and their analogues,purines and pyrimidines and their derivatives,lipids and lipid-like molecules.There are 5 metabolic pathways related to enteritis in rat serum,namely arginine biosynthesis,D-arginine and D-ornithine metabolism,phenylalanine,tyrosine and tryptophan biosynthesis,phenylalanine metabolism,arginine and proline metabolism.The results indicate that JFP-Ps can attenuate inflammation by regulating amino acid metabolism and influencing related pathways in rats with DSS-induced colitis.

关 键 词：菠萝蜜多糖 肠炎大鼠 血液 超高液相串联四极杆飞行时间质谱 代谢组学 

分 类 号：TS201.4[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]