黑灵芝多糖对脂多糖诱导的IEC-6肠上皮细胞损伤的保护作用  

Protective Effect of Ganoderma atrum Polysaccharide on Lipopolysaccharide-induced Damagein Intestinal Epithelial Cell IEC-6

作　　者：郑冰 胡晓波[1] 陈奕[1] 谢建华[1] 余强[1] Zheng Bing;Hu Xiaobo;Chen Yi;Xie Jianhua;Yu Qiang(State Key Laboratory of Food Science and Resources,China-Canada Joint Lab of Food Science and Technology(Nanchang),Nanchang University,Nanchang 330047)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室中加食品科学技术联合实验室(南昌),南昌330047

出　　处：《中国食品学报》2024年第4期43-53,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区科学基金项目(81760737)。

摘　　要：目的:探究黑灵芝多糖(PSG-1)对脂多糖(LPS)诱导的IEC-6肠上皮细胞损伤的保护作用及其机制。方法:采用LPS构建肠上皮细胞IEC-6损伤模型,研究PSG-1对IEC-6细胞的干预效果。采用细胞计数盒(cck-8)法测定PSG-1干预对细胞活力的影响。运用western-blot技术探究细胞中肠道紧密连接蛋白和环氧化酶cox-2表达的变化,基于转录组测序技术分析PSG-1潜在的保护机制并对其进行验证。结果:PSG-1干预可以显著提升LPS造成的细胞活力降低和肠道紧密连接蛋白ZO-1、Claudin-1和Occludin的表达,而且PSG-1对LPS引起的cox-2异常高表达具有抑制效果。转录组测序及划痕试验和蛋白免疫印迹试验结果表明:PSG-1能显著增强细胞的迁移能力并抑制促凋亡蛋白Bax、Caspase-3和Caspase-9的表达。结论:PSG-1对LPS诱导的肠上皮细胞IEC-6具有显著的保护作用,细胞迁移和凋亡可能是PSG-1发挥其保护效应的关键途径。Objective:To investigate the protective effect of Ganoderma atrum polysaccharide(PSG-1)against lipopolysaccharide(LPS)-induced damage in intestinal epithelial cell(IEC-6)and its underlying mechanisms.Methods:The intervention effects of PSG-1 on IEC-6 cells were investigated using a LPS-induced intestinal epithelial cell IEC-6 injury model,and the effect of PSG-1 intervention on cell viability was assessed using the cell counting kit-8(CCK-8)assay.Western blot technology was employed to investigate the alterations in the expression of intestinal tight junction proteins and cyclooxygenase-2(cox-2)in the cells.Transcriptome sequencing analysis was conducted to explore the potential protective mechanisms of PSG-1,followed by experimental validation.Results:Intervention with PSG-1 significantly enhanced cell viability and the expression of intestinal barrier proteins,including ZO-1,Claudin-1,and Occludin.Moreover,PSG-1 exhibited an inhibitory effect on the abnormal upregulation ofcox-2 induced by LPS.Transcriptome sequencing analysis,scratch assay,and western blot experiments validation demonstrated that PSG-1 significantly enhanced cell migration ability while suppressing the expression of pro-apoptotic proteins,including Bax,Caspase-3,and Caspase-9.Conclusion:PSG-1 exhibited significant improvement in LPS-induced damage in IEC-6 cell.Cell migration and apoptosis might be key mechanisms through which PSG-1 exerted its protective effects.

关 键 词：黑灵芝多糖 脂多糖 肠上皮细胞IEC-6 细胞迁移 凋亡 

分 类 号：TS201.4[轻工技术与工程—食品科学]